Vinculin anticorps (C-Term)

N° du produit ABIN6992009

L’anticorps anti-Vinculin Polyclonal Lapin est utilisé pour la détection de Vinculin dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IF et IHC (p).

Aperçu rapide pour Vinculin anticorps (C-Term) (ABIN6992009)

Antigène: Vinculin (VCL)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Vinculin est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-Vinculin anticorps

Épitope: AA 1060-1110, C-Term

Specificité: Vinculin antibody is human, mouse and rat specific. At least three isoforms of Vinculin are known to exist.

Purification: Vinculin Antibody is affinity chromatography purified via peptide column.

Immunogène: Anti-Vinculin antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human Vinculin. The immunogen is located within amino acids 1060-1110 of Vinculin.

Isotype: IgG

Information d'application

Indications d'application: WB: 1-2 μ,g/mL, IHC: 5 μ,g/mL, IF: 20 μ,g/mL.

Antibody validated: Western Blot in human, mouse and rat samples, Immunohistochemistry in rat samples, Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: Vinculin Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: Vinculin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Détails sur Vinculin

Antigène: Vinculin (VCL)

Autre désignation: Vinculin

Sujet: Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2,3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).

Poids moléculaire: Predicted: 124kD

Observed: 124kD kDa

ID gène: 7414

NCBI Accession: NP_003364

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2