ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Specificité
IQSEC2 antibody is human, mouse and rat reactive. At least two isoforms of IQSEC2 are known to exist, this antibody will only detect the larger isoform. IQSEC2 antibody is predicted to not cross-react with IQSEC1.
Purification
IQSEC2 antibody is affinity chromatography purified via peptide column.
Immunogène
IQSEC2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IQSEC2. The immunogen is located within amino acids 1380 - 1430 of IQSEC2.
IQSEC2
Reactivité: Souris
IF (cc), IF (p)
Hôte: Lapin
Polyclonal
Alexa Fluor 647
Indications d'application
IQSEC2 antibody can be used for detection of IQSEC2 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 5 μ,g/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
IQSEC2 antibody is supplied in PBS containing 0.02 % sodium azide.
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
-20 °C,4 °C
Stockage commentaire
IQSEC2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
ADP ribosylation factors (Arfs) are small GTP-binding proteins known for their role in vesicular transport, where they nucleate the assembly of coat protein complexes at sites of carrier vesicle formation. Similar to the related protein IQSEC1, IQSEC2 is a guanine nucleotide exchange protein for ARF1 and ARF6 that belongs to the BRAG family and contains one IQ domain, one PH domain and one SEC7 domain (1,2). IQSEC2 is a major component of the postsynaptic density (PSD) and colocalizes with PSD-95. It has been suggested to modulate the functions of the ARF proteins (1). Mutations in this gene have been implicated in nonsyndromic X-linked mental retardation (3).