LAG3 anticorps (N-Term)

N° du produit ABIN6992196

Détail du produit anti-LAG3 anticorps

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Épitope: AA 110-160, N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LAG3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificité: At least two antibodies are known to exist, this antibody will only detect the largest isoform.

Purification: LAG3 Antibody is affinity chromatography purified via peptide column.

Immunogène: LAG3 antibody was raised against a peptide corresponding to 18 amino acids near the amino terminus of human LAG3. The immunogen is located within amino acids 110 - 160 of LAG3.

Isotype: IgG

Information d'application

Indications d'application: LAG3 antibody can be used for Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 5 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in mouse samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer: LAG3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C,4 °C

Stockage commentaire: LAG3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur LAG3

Antigène: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Autre désignation: LAG3 (LAG3 Produits)

Synonymes: anticorps CD223, anticorps LAG-3, anticorps Ly66, anticorps lymphocyte activating 3, anticorps lymphocyte-activation gene 3, anticorps LAG3, anticorps Lag3

Sujet: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Poids moléculaire: Predicted: 55 kDa

Observed: 54 kDa

ID gène: 3902

NCBI Accession: NP_002277

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints