Histone H4 anticorps (AA 2-103)

N° du produit ABIN7440314

Cet validé indépendamment anticorps Lapin Polyclonal détecte spécifiquement Histone H4 dans WB, IHC, ICC et IP. Il présente une réactivité envers Humain, Souris, Rat, Porc, Boeuf (Vache), Poulet, Cat, Chien, Cobaye, Cheval et Macaque rhésus.

Aperçu rapide pour Histone H4 anticorps (AA 2-103) (ABIN7440314)

Antigène: Histone H4

Reactivité: Humain, Souris, Rat, Porc, Boeuf (Vache), Poulet, Cat, Chien, Cobaye, Cheval, Macaque rhésus

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Histone H4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Détail du produit anti-Histone H4 anticorps

Épitope: AA 2-103

Fonction: Polyclonal Antibody to Histone H4 (H4)

Specificité: The antibody is a rabbit polyclonal antibody raised against H4. It has been selected for its ability to recognize H4 in immunohistochemical staining and western blotting.

Réactivité croisée: Cat, Poulet, Boeuf (Vache), Chien, Cobaye, Cheval, Humain, Souris, Porc, Lapin, Rat, Macaque rhésus

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogène: Recombinant Histone H4 (H4) corresdonding to Ser2~Gly103 with N-terminal His and GST Tag

Isotype: IgG

Information d'application

Indications d'application: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Commentaires:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Independent Validation (IHC)

Validation #104631 (Immunohistochemistry)

by: Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin

No.: #104631

Date: 04.08.2025

Antigène: Histone H4

Numéro du lot: A20250716116

Application validée: Immunohistochemistry

Contrôle positif:

Contrôle négative:

Control slices were processed for each experimental procedure, omitting the primary antibody. In this case, overnight incubation was only performed using a diluent solution.

Validation Images

Histone H4 immunoreactive cells in the cerebral cortex and hippocampus of an adult CD1 mouse. Antibody dilution: 1:25. Magnification: 63x.

Protocole

Anticorps primaire: ABIN7440314

Anticorps secondaire: anti-rabbit Alexa Fluor 488 (Invitrogen A11008, lot # 3112790)

Full Protocol:

• Sample analyzed: adult (9 months old) CD1 mouse brain.
• Paraffin-embedded slices (7µm thick) were deparaffinized and rehydrated through a graded series of alcohols and distilled water (dH2O).
• Sections were blocked in ovalbumin 1% 1 hour at room temperature.
• Antigen retrieval was performed in all the sections by microwave treatment (1 minute at 750W + 1 minute at 250W in Citrate buffer pH 6).
• 3x5 minutes washing in 0.01M PBS.
• Sections were incubated with the primary antibody at the following dilutions of 1:25/1:50/1:100/1:200, overnight at room temperature (primary antibody was diluted in PLL/BSA/PBS diluent).
• 3x5 minutes washing in 0.01M PBS.
• Incubation with the anti-rabbit secondary antibody 1:400 in PLL/BSA/PBS diluent, 1 hour at room temperature.
• 3x5 minutes washing in 0.01M PBS.
• Nuclear counterstaining was performed with 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) 1µg/mL, 2 minutes.
• 2x5 minutes washing in 0.01M PBS.
• Specimens were then mounted in Fluoroshield mounting medium (Sigma).
• Images were acquired with a Leica DM 6000B fluorescence microscope with a digital camera at 63x magnification.

Notes:

Passed. The antibody works in IHC-P at concentrations from 1:25-1:50.

Stockage

Format: Liquid

Concentration: 0.33 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Date de péremption: 24 months

Détails sur Histone H4

Antigène: Histone H4