Cathepsin B anticorps (Internal Region)

N° du produit ABIN7013865

Cet anticorps Chèvre Polyclonal détecte spécifiquement Cathepsin B dans WB, IHC, ELISA, FACS et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour Cathepsin B anticorps (Internal Region) (ABIN7013865)

Antigène: Cathepsin B (CTSB)

Reactivité: Humain

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Cathepsin B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Classe de qualité: Verified

Détail du produit anti-Cathepsin B anticorps

Épitope: Internal Region

Fonction: Cathepsin B

Séquence: SDRICIHTNA HVSVE

Specificité: Reported variants represent identical protein: NP_680091.1, NP_680090.1, NP_680092.1, NP_001899.1, NP_680093.1.

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 5 μg/mL.

Western Blot: Approx 35 kDa band observed in lysates of cell line HepG2 and in Human Lung, Human Colo-rectal carcinoma and Human Brain Glio-Blastoma cancer lysates (calculated MW of 37.8x kDa according to NP_001899.1). Recommended concentration: 0.5-2 μg/m

Peptide ELISA: antibody detection limit dilution 1:64000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ vesicles of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration:

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur Cathepsin B

Antigène: Cathepsin B (CTSB)

Autre désignation: CTSB

Sujet: CTSB, cathepsin B, APPS, CPSB, APP secretase, amyloid precursor protein secretase, cathepsin B1, cysteine protease

ID gène: 1508

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades