EDF1 anticorps (N-Term)

N° du produit ABIN7013866

L’anticorps Chèvre Polyclonal anti-EDF1 a été validé pour WB, IHC, ELISA, IF et FACS. Il convient pour détecter EDF1 dans des échantillons de Humain et Souris.

Aperçu rapide pour EDF1 anticorps (N-Term) (ABIN7013866)

Antigène: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EDF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-EDF1 anticorps

Épitope: N-Term

Fonction: EDF1 / MBF1

Séquence: AESDWDTVTV LRK

Réactivité croisée: Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Pancreas and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 19 kDa band observed in nuckear lysates of cell line HEK293, approx. 18 kDa in nuckear lysates of cell line HeLa, and approx 17 kDa in nuckear lysates of cell line NIH3T3 (calculated MW of 16.4 kDa according to Human NP_003783.1 and Mous

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the nuclei of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10u

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur EDF1

Antigène: EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))

Autre désignation: EDF1

Sujet: EDF1, MBF1, EDF-1, MGC9058, endothelial differentiation-related factor 1, multiprotein bridging factor 1

ID gène: 8721, 59022