EHD1 anticorps (N-Term)

N° du produit ABIN7013871

Cet anticorps Chèvre Polyclonal détecte spécifiquement EHD1 dans WB, ELISA, IHC, IF et FACS. Il présente une réactivité envers Humain et Souris.

Aperçu rapide pour EHD1 anticorps (N-Term) (ABIN7013871)

Antigène: EHD1 (EH-Domain Containing 1 (EHD1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp EHD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Classe de qualité: Verified

Détail du produit anti-EHD1 anticorps

Épitope: N-Term

Fonction: EHD1

Séquence: FSWVSKDARR KKEPC

Réactivité croisée: Boeuf (Vache), Humain, Souris, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Information d'application

Indications d'application:

Immunohistochemistry: Paraffin embedded Human Thymus and Brain (Cortex). Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 60 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 60.6 kDa according to Mouse NP_0342491) Preliminary testing also showed a band at approx 60 kDa in Human Lymph node and Rat Kidney lysate and in HeLa cell lysate. Rec

Peptide ELISA: antibody detection limit dilution 1:32000.

Commentaires:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended c

Restrictions: For Research Use only

Stockage

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur EHD1

Antigène: EHD1 (EH-Domain Containing 1 (EHD1))

Autre désignation: EHD1

Sujet: EHD1, PAST, PAST1, H-PAST, HPAST1, EH-domain containing 1, testilin, FLJ42622, FLJ44618, OTTHUMP00000069747

ID gène: 10938

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development