CASR anticorps (Extracellular, N-Term) (FITC)

N° du produit ABIN7043019

Cet anticorps anti-CASR Polyclonal Lapin conjugué à FITC (ABIN7043019) détecte spécifiquement CASR dans FACS et LCI. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour CASR anticorps (Extracellular, N-Term) (FITC) (ABIN7043019)

Antigène: CASR (Calcium-Sensing Receptor (CASR))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CASR est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-CASR anticorps (FITC)

Épitope: AA 216-229, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor Conjugated to the Fluorescent Dye FITC

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat,mouse,bovine,dog - identical

Attributs du produit: Anti-Calcium Sensing Receptor (extracellular) Antibody (ABIN7043018, ABIN7044161 and ABIN7044162) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry applications. It has been designed to recognize CaSR from human, rat, and mouse samples. \nAnti-Calcium Sensing Receptor (extracellular)-FITC Antibody (ABIN7043018, ABIN7044161 and ABIN7044162)-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry using live cells.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

Isotype: IgG

Information d'application

Indications d'application:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235016)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CASR

Antigène: CASR (Calcium-Sensing Receptor (CASR))

Autre désignation: CASR

Sujet:

Synonyms: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Description: The calcium-sensing receptor (CaSR) is a class C G-protein-coupled receptor encoded by a single copy gene. CaSR plays a major role in the maintenance of a physiological serum ionized Ca2+ concentration by regulating the circulating levels of parathyroid hormone1.The N-terminus of the receptor is comprised of a signal peptide of 19 amino acids. This terminus directs the nascent CaSR polypeptide into the lumen of the endoplasmic reticulum (ER) and is then cleaved. The extracellular domain (ECD) of the mature protein has approximately 600 amino acids, comprised of a bi-lobed Venus-flytrap-like domain (VFT) connected by a cysteine-rich region and a short peptide linker to the seven transmembrane domain (TMD) with an intracellular C-terminal tail of 216 amino acids. CaSR functions as a homodimer with both covalent and noncovalent interactions between the VFT domains of each monomer. Ca2+ binds in the cleft between the two lobes of each VFT causing the lobes to close on one another and the VFT to rotate with transfer of a conformational change to the cysteine-rich region.In the parathyroid gland, up-regulation of CaSR by 1,25-dihydroxyvitamin D [1,25(OH)2D] makes the gland more responsive to extracellular Ca2+ and parathyroid hormone (PTH) secretion is reduced. While somatic mutations in CASR are rare, parathyroid glands from patients with primary or severe uremic secondary hyperparathyroidism have reduced CaSR expression2.

ID gène: 846

NCBI Accession: NM_000388

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis