CATSPER1 anticorps (3rd Extracellular Loop)

N° du produit ABIN7043027

L’anticorps anti-CATSPER1 Polyclonal Lapin est utilisé pour la détection de CATSPER1 dans des échantillons de Souris. Il a été validé pour WB.

Aperçu rapide pour CATSPER1 anticorps (3rd Extracellular Loop) (ABIN7043027)

Antigène: CATSPER1 (Cation Channel, Sperm Associated 1 (CATSPER1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CATSPER1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-CATSPER1 anticorps

Épitope: 3rd Extracellular Loop, AA 508-521

Fonction: A Rabbit Polyclonal Antibody to CatSper1 Channel

Réactivité croisée: Souris, Rat

Homologie: Rat,human - 12,14 amino acid residues identical

Attributs du produit: Anti-CatSper1 (extracellular) Antibody (ABIN7043027 and ABIN7044102) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting CatSper1 in living cells. It has been designed to recognize CatSper1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RALFQDSDPKRFQN, corresponding to amino acid residues 508-521 of mouse CatSper1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235036)

Blocking Peptide: (ABIN7235036)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CATSPER1

Antigène: CATSPER1 (Cation Channel, Sperm Associated 1 (CATSPER1))

Autre désignation: CATSPER1

Sujet:

Synonyms: Cation channel sperm-associated protein 1

Description: Capacitation is the final stage of spermatozoon maturation which eventually enables it to bind the zona pellucida (ZP) - a thick layer of glycoproteins surrounding the oocyte1-3. It occurs in the fallopian tubes of the female reproductive tract and is preceded by spermatogenesis (testes) and epididymal maturation (epididymis)3,4. Capacitation is accompanied by drastic morphological and functional alterations, such as membrane hyperpolarization, changes in membrane lipid composition, intracellular alkalinization, increased level of protein tyrosine phosphorylation by cAMP-dependent PKA, and elevated intracellular concentrations of Ca2+ ([Ca2+]i) and pH i1,2, which render the sperm chemotaxis-responsive and hyperactive6. Increased flagellar bend, triggered by Ca2+ signaling, produces asymmetrical, whiplash-like motility pattern that provides the sperm with significantly stronger swimming thrust1-3. Hyperactivity, together with the eruption of hydrolytic enzymes from the sperm's acrosome vesicle facilitate the disruption of the ZP matrix and make way for the sperm to enter the egg cytoplasm1-7. CatSper (cation channel of spermatozoa) is a sperm-specific Ca2+ channel located explicitly in the spermatozoon flagellum2. It mediates flagellar hyperactivity7, and is chemotactically responsive to progesterone8. CatSper is composed of 4 pore-forming subunits (CatSper1-4) with 6 transmembrane (TM) domains each, and of 3 auxiliary subunits: CatSperβ (2 TM domains), CatSperγ and CatSperδ (1 TM domain each)1, 2. While all four CatSper proteins are necessary for the channel's function (and hence male fertility)9, each is synthesized independently. Trafficking to the flagellum requires the cooperation of all subunits1,3.CatSper1, the first identified subunit of the channel, is highly homologous to CaV channels, a voltage sensor domain (S1-S4) containing enough positively charged residues to confer weak voltage sensitivity to the channel2. Furthermore, its histidine-rich, intracellular amino terminus likely serves as the pH sensor necessary to induce hyperactivation (by permitting an influx of Ca2+)3. Indeed, intracellular alkaline pH (7.9-8.5) was shown to efficiently hyper-activate CatSper channels6.

ID gène: 225865

NCBI Accession: NM_053054

UniProt: Q91ZR5