CATSPER4 anticorps (1st Extracellular Loop)

N° du produit ABIN7043028

Cet anticorps anti-CATSPER4 Polyclonal Lapin (ABIN7043028) détecte spécifiquement CATSPER4 dans WB, IF et IC. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour CATSPER4 anticorps (1st Extracellular Loop) (ABIN7043028)

Antigène: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CATSPER4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-CATSPER4 anticorps

Épitope: 1st Extracellular Loop, AA 90-105

Fonction: A Rabbit Polyclonal Antibody to CatSper4 Channel

Réactivité croisée: Souris, Rat

Homologie: Rat - identical, human -15,16 amino acid residues identical

Attributs du produit: Anti-CatSper4 (extracellular) Antibody is directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (ABIN7043028, ABIN7044103 and ABIN7044104) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:1000

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Cited Application: ICC

Negative Control: (ABIN7235039)

Blocking Peptide: (ABIN7235039)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CATSPER4

Antigène: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Autre désignation: CATSPER4

Sujet:

Synonyms: Cation channel sperm-associated protein 4

Description: During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence TxDxW indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH - and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.

ID gène: 329954

NCBI Accession: NM_198137

UniProt: Q8BVN3