CCR9 anticorps (2nd Extracellular Loop)

N° du produit ABIN7043036

L’anticorps anti-CCR9 Polyclonal Lapin est utilisé pour la détection de CCR9 dans des échantillons de Humain. Il a été validé pour WB, FACS et LCI.

Aperçu rapide pour CCR9 anticorps (2nd Extracellular Loop) (ABIN7043036)

Antigène: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCR9 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-CCR9 anticorps

Épitope: 2nd Extracellular Loop, AA 205-218

Fonction: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,rat - 10,14 amino acid residues identical

Attributs du produit: Anti-CCR9 (extracellular) Antibody (ABIN7043036, ABIN7044177 and ABIN7044178) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR9 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)DESTKLKSAVLTLK, corresponding to amino acid residues 205-218 of human CCR9

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235059)

Blocking Peptide: (ABIN7235059)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CCR9

Antigène: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Autre désignation: CCR9

Sujet:

Synonyms: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199

Description: CCR9 (C-C chemokine receptor type 9) belongs to the β-chemokine receptor family of the G-protein coupled receptor superfamily. Chemokine receptors play a crucial role in cell migration and are considered to be important therapeutic targets for inflammatory diseases and in tumor chemoresistance and metastasis. In addition, high expression of CCR9 has been reported in a wide range of cancers including melanoma, ovarian cancer, lung cancer, breast cancer and colon cancer. Thus, CCR9 is considered to be a potential tumor biomarker in diagnosis and therapy of cancer1,2. Under physiological conditions, CCR9 is mainly expressed in immature T lymphocytes and on the surface of intestinal cells2.CCR9 receptor interacts with its specific ligand- chemokine ligand 25 (CCL25, thymus-expressed chemokine) in a two-step binding mechanism in which the C-terminal region of CCL25 first binds the N-terminus region and extracellular loops of the receptor followed by the binding of the N-terminus of the chemokine. The two-step biding mechanism stabilizes the receptor in an active conformation that facilitates intracellular signaling1.

ID gène: 10803

NCBI Accession: NM_001256369

UniProt: P51686