CX3CL1 anticorps (Extracellular, N-Term)

N° du produit ABIN7886258

L’anticorps anti-CX3CL1 Polyclonal Lapin est utilisé pour la détection de CX3CL1 dans des échantillons de Humain. Il a été validé pour WB, IHC et IF.

Aperçu rapide pour CX3CL1 anticorps (Extracellular, N-Term) (ABIN7886258)

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CX3CL1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-CX3CL1 anticorps

Épitope: AA 58-71, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to CX3CL1

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - identical, mouse - 12,14 amino acid residues identical

Attributs du produit: Anti-CX3CL1 (extracellular) Antibody (ABIN7043045, ABIN7044192 and ABIN7044193) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize CX3CL1 from human, mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: CGKRAIILETRQHR, corresponding to amino acid residues 58-71 of human CX3CL1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235071)

Blocking Peptide: (ABIN7235071)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CX3CL1

Antigène: CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))

Autre désignation: CX3CL1

Sujet:

Synonyms: Fractalkine, C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1, FKN, NTT, SCYD1

Description: CX3C chemokine ligand 1 (CX3CL1), also known as fractalkine (FKN), is a chemokine belonging to the CX3C family. CX3CL1 is secreted by neurons and plays an important role in modulating glial activation in the central nervous system after binding to its sole receptor CX3CR1 which mainly is expressed on microglia. The CX3CL1/CX3CR1 interaction is an important bridge connecting neuron and microglia1.CX3CL1 is a large cytokine protein with an extended mucin-like stalk and a chemokine domain at the N-terminus. It is the only member of CX3C family which belongs to the large family of small secreted chemotactic cytokines. CX3CL1 is highly and constitutively expressed in hippocampal and cortical neurons. It exists in both secreted and membrane-bound form. While the secreted form contains only the chemokine domain located in the N-terminus, the membrane-bound form contains the chemokine domain, a mucin-like stalk, a transmembrane domain and an intracellular C-terminal domain. The membrane-tethered mucin stalk acts as a cell adhesion molecule adhering to microglia during an inflammatory reaction, it can be cleaved to produce a soluble glycoprotein by metalloproteinases ADAM 10 and ADAM 17 or lysosomal cysteine protease and cathepsin S (CatS), The soluble form has been proposed to act as a chemoattractant and can serve as a signaling molecule mediating neural/microglial interactions via its receptor CX3CR1, mainly expressed on microglia and partly on astrocyte as well as on neurons in the CNS. The CX3CL1/CX3CR1 interaction on microglia is likely to alter the microglial state to a more neuroprotective one. Notably, transmembrane and soluble CX3CL1 elicit different cytokine responses in immune cells1-3.CX3CL1 has been associated with learning and memory as CX3CL1 is upregulated in the rat hippocampus during memory-associated synaptic plasticity. It is considered as a potent neuromodulator of the evoked excitatory synaptic transmission and plays a major role in synaptic plasticity and neuroprotection1,4.

ID gène: 6376

NCBI Accession: NM_002996

UniProt: P78423

Pathways: Synaptic Membrane