CLCN2 anticorps (Intracellular)

N° du produit ABIN7043051

L’anticorps Lapin Polyclonal anti-CLCN2 a été validé pour WB, IHC, IF et IC. Il convient pour détecter CLCN2 dans des échantillons de Rat.

Aperçu rapide pour CLCN2 anticorps (Intracellular) (ABIN7043051)

Antigène: CLCN2 (Chloride Channel 2 (CLCN2))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CLCN2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Classe de qualité: KO Validated

Détail du produit anti-CLCN2 anticorps

Épitope: AA 888-906, Intracellular

Fonction: A Rabbit Polyclonal Antibody to CLC-2 (CLCN2) Channel

Specificité: Intracellular, C-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: human - 17, guinea pig - 15,swine - 18,19 amino acid residues identical,Mouse - identical, rabbit

Attributs du produit: Anti- (CLCN2) Antibody (ABIN7043051 and ABIN7044123) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize channel from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: RSRHGLPREGTPSDSDDKC, corresponding to amino acid residues 888-906 of rat CLC-2.

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235082)

Blocking Peptide: (ABIN7235082)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur CLCN2

Antigène: CLCN2 (Chloride Channel 2 (CLCN2))

Autre désignation: CLCN2

Sujet: Chloride channel 2, ClC2,CLC-2 is a member of the voltage-dependent Cl- channel (CLC) family that includes nine known members in mammals. CLC channels can be classified as plasma membrane channels and intracellular organelle channels. The first group includes the CLC-1, CLC-2 CLC-Ka and CLCKb channels. The second group comprises the CLC-3, CLC-4, CLC-5, CLC-6 and CLC-7.CLC channels that function in the plasma membrane are involved in the stabilization of membrane potential and in transepithelial transport. The presumed function of the intracellular CLC channels is support of the acidification of the intraorganellar compartment. In this regard, recent reports indicate that ClC-4 and ClC-5 (and by inference ClC-3) can function as Cl-/H+ antiporters.1, 2The functional unit of the CLC channels is a dimer with each subunit forming a proper pore. Although the crystal structure of bacterial CLC channels was resolved, the topology of the CLC channels is complex and has not been fully elucidated. It is generally accepted that both the N- and C- terminus domains are intracellular while the number and configuration of the transmembrane domains vary greatly between different models. 1,2CLC-2 is widely distributed with prominent expression in brain, kidney, lung and the gastrointestinal system. Mutations in the CLC-2 channel gene detected in humans are associated with idiopathic generalized epilepsies while disruption of the ClC-2 gene in mice is associated with testicular and retinal degeneration. 3,4

Alternative names: CLC-2 (CLCN2), Chloride channel 2, ClC2

ID gène: 29232

NCBI Accession: NM_001171087

UniProt: P35525