CLIC4 anticorps (Intracellular)

N° du produit ABIN7043057

L’anticorps Lapin Polyclonal anti-CLIC4 a été validé pour WB, IF et IC. Il convient pour détecter CLIC4 dans des échantillons de Humain.

Aperçu rapide pour CLIC4 anticorps (Intracellular) (ABIN7043057)

Antigène: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CLIC4 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-CLIC4 anticorps

Épitope: AA 8-20, Intracellular

Fonction: A Rabbit Polyclonal Antibody to CLIC4 Channel

Specificité: Intracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat,mouse - identical

Attributs du produit: Anti-CLIC4 Antibody (ABIN7043057 and ABIN7044140) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC4 from mouse, rat, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGLKEEDKEPLIE, corresponding to amino acid residues 8-20 of human CLIC4

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235106)

Blocking Peptide: (ABIN7235106)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur CLIC4

Antigène: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Autre désignation: CLIC4

Sujet: Cl- intracellular channel 4, Intracellular chloride ion channel p64H1,Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1. For instance, CLIC4 is involved in keratinocyte differentiation in a TGFb-dependent manner4,5, in such a way that TGFb stimulation induces the expression and nuclear localization of CLIC4 and its subsequent interaction with transcription factors4. CLIC4 is also required for growth arrest and apoptosis under stress6.CLIC4 distribution is rather ubiquitous but shows higher expression level in the skin, kidney, brain and testes7.

Alternative names: CLIC4, Cl- intracellular channel 4, Intracellular chloride ion channel p64H1

ID gène: 25932

NCBI Accession: NM_013943

UniProt: Q9Y696