CHRM5 anticorps (Extracellular)

N° du produit ABIN7043065

Cet anticorps Lapin Polyclonal détecte spécifiquement CHRM5 dans WB, FACS et LCI. Il présente une réactivité envers Humain.

Aperçu rapide pour CHRM5 anticorps (Extracellular) (ABIN7043065)

Antigène: CHRM5 (Cholinergic Receptor, Muscarinic 5 (CHRM5))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CHRM5 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-CHRM5 anticorps

Épitope: AA 7-24, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Muscarinic Acetylcholine Receptor M5

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Rat

Homologie: 18 amino acid residues identical,Mouse - 16, rat - 14

Attributs du produit: Anti-CHRM5 (extracellular) Antibody is directed against an extracellular epitope of the human M5 muscarinic receptor. Anti-CHRM5 (extracellular) Antibody (ABIN7043065, ABIN7044586 and ABIN7044587) can be used for western blot analysis. It was specifically designed to recognize rat, mouse and human M5.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HNATTVNGTPVNHQPLER, corresponding to amino acid residues 7-24 of human M5 muscarinic receptor

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235129)

Blocking Peptide: (ABIN7235129)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur CHRM5

Antigène: CHRM5 (Cholinergic Receptor, Muscarinic 5 (CHRM5))

Autre désignation: CHRM5

Sujet: Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5,Acetylcholine is a neurotransmitter which activates two different groups of receptors: nicotinic acetylcholine receptors (nAChR) which belong to the superfamily of ligand-gated ion channels, and muscarinic acetylcholine receptors (mAChR) which belong to the G-protein coupled receptor (GPCR) superfamily. There are five separate gene products (M1-M5) that, assembled, make up this GPCR subfamily and like all GPCRs, have seven transmembrane spanning domains.Generally, M1, M3 and M5 muscarinic receptors are known to activate phospholipase C (PLC) via Gq coupling, while M2 and M4 muscarinic receptors couple to Gi/o, and therefore inhibit adenylate cyclase1. This classification is however not clear cut.mAChR can activate adenylate cyclase by coupling to Gs2. In addition, M2 and M4 muscarinic receptors, when overexpressed, can also activate adenylate cyclase in some systems3-5.Evidence suggests that muscarinic receptors can form homo- or heterodimers and that the dimer formed can subsequently affect the downstream signaling pathways1.Muscarinic receptors have been shown to regulate voltage-gated Ca2+ channels, namely CaV2.16, as well acid sensing ion channels (ASIC)7. Generally speaking, the actions of muscarinic receptors on ion channels can be either via 2nd messengers or through their direct action on the channels once activated8.Furthermore, muscarinic receptors can also promote endocytosis of ion channels (KV1.2 for example) by recruiting tyrosine kinases that phosphorylate the channel in order to terminate its activity9. Other diverse and important functions of muscarinic receptors include cell growth, survival and physiology1.Expression of muscarinic receptors is found in neurons in the central nervous system as well as in the peripheral nervous system. These receptors are also expressed in cardiac and smooth muscle, lung, intestine, ovary and urothelium1,10.The M5 muscarinic receptor has been the most difficult receptor to study as there is no specific pharmacology targeted against the receptor and no high level of expression has been detected in tissues. However, the effects of single knockout mice were studied and it was observed that in M5 knockouts there is a moderate decrease in ligand binding in the lung and bladder, two organs expressing the M5 muscarinic receptor11. In addition, it has been proposed that the M5 muscarinic receptor may be involved in interstitial cystitis, a urinary bladder disorder12.

Alternative names: M5 Muscarinic Receptor, CHRM5, Muscarinic acetylcholine receptor M5, Cholinergic receptor muscarinic 5, mAChR M5, HM5

ID gène: 1133

NCBI Accession: NM_001320917

UniProt: P08912

Pathways: Synaptic Membrane