CysLTR1 anticorps (3rd Extracellular Loop)

N° du produit ABIN7043093

L’anticorps anti-CysLTR1 Polyclonal Lapin est utilisé pour la détection de CysLTR1 dans des échantillons de Humain. Il a été validé pour WB et FACS.

Aperçu rapide pour CysLTR1 anticorps (3rd Extracellular Loop) (ABIN7043093)

Antigène: CysLTR1 (CYSLTR1) (Cysteinyl Leukotriene Receptor 1 (CYSLTR1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CysLTR1 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-CysLTR1 anticorps

Épitope: 3rd Extracellular Loop, AA 261-275

Fonction: A Rabbit Polyclonal Antibody to CysLTR1

Réactivité croisée: Humain, Souris

Homologie: mouse - 12,Human - 14,15, rat - 11,15 amino acid residues identical

Attributs du produit: Anti-CysLTR1 (extracellular) Antibody (ABIN7043093, ABIN7044558 and ABIN7044559) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR1 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 1:50

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235196)

Blocking Peptide: (ABIN7235196)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur CysLTR1

Antigène: CysLTR1 (CYSLTR1) (Cysteinyl Leukotriene Receptor 1 (CYSLTR1))

Autre désignation: CYSLTR1

Sujet:

Synonyms: CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81

Description: LTC4, LTD4, and LTE4 are leukotrienes (cys-LTs) derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4 whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

ID gène: 10800

NCBI Accession: NM_001282186

UniProt: Q9Y271