Ephrin A2 anticorps (Extracellular)

N° du produit ABIN7043129

L’anticorps Lapin Polyclonal anti-Ephrin A2 a été validé pour WB, IF, FACS, IHC et LCI. Il convient pour détecter Ephrin A2 dans des échantillons de Souris.

Aperçu rapide pour Ephrin A2 anticorps (Extracellular) (ABIN7043129)

Antigène: Ephrin A2 (EFNA2)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ephrin A2 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Détail du produit anti-Ephrin A2 anticorps

Épitope: AA 71-84, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Ephrin-A2

Specificité: Extracellular

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - identical, human - 13,14 amino acid residues identical

Attributs du produit: Anti-Ephrin-A2 (extracellular) Antibody (ABIN7043129, ABIN7044257 and ABIN7044258) is a highly specific antibody directed against an epitope of mouse EFNA2. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry. It has been designed to recognize EFNA2 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)HYGAPLPPAERMER, corresponding to amino acid residues 71-84 of mouse Ephrin-A2

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:400

Commentaires:

Negative Control: (ABIN7235305)

Blocking Peptide: (ABIN7235305)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur Ephrin A2

Antigène: Ephrin A2 (EFNA2)

Autre désignation: EFNA2

Sujet: EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2,Eph receptor tyrosine kinases family and their ligands, ephrins, are membrane proteins that primarily regulate cell to cell repulsion and cell adhesion and movement by modulating the organization of the actin cytoskeleton mainly through Rho family GTPases. Eph receptors and ephrin ligand signaling regulaes many aspects of cell interaction in the developing neocortex, including migration and axonal target recognition. Ephrin signaling plays an important role in guiding neurons and their projections during embryonic development1,2.In mammals, the Eph receptor tyrosine kinases family has 14 members that are divided into 2 subclasses, the EphA (A1-A8 and A10) and EphB (B1-B4 and B6). This division is based on the sequence similarity of their extracellular domains. EphA receptors bind to glycosylphosphatidyl inositol-anchored ephrin-A ligands, and EphB receptors bind to transmembrane ephrin-B proteins. The ephrin-A proteins include five members ephrin-A1 to ephrin-A5 whereas ephrin-B proteins include three ephrin-B1 to ephrin-B32,3.Ephrin-A2 protein is abundantly expressed in retinal axons. Mutations in Ephrin-A2 disrupts topographic ordering of projections in the developing visual system. A knockout of ephrin-A2 in adult mice shows focal patches of disorganized neocortical laminar architecture, ranging in severity from reduced neuronal density to a complete lack of neurons1,2.

Alternative names: Ephrin-A2, EPH-related receptor tyrosine kinase ligand 6, LERK6, CEK7-ligand, EFNA2

ID gène: 13637

NCBI Accession: NM_001405

UniProt: P52801