FFAR2 anticorps (2nd Extracellular Loop)

N° du produit ABIN7043139

Cet anticorps anti-FFAR2 Polyclonal Lapin (ABIN7043139) détecte spécifiquement FFAR2 dans WB, FACS et LCI. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour FFAR2 anticorps (2nd Extracellular Loop) (ABIN7043139)

Antigène: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FFAR2 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-FFAR2 anticorps

Épitope: 2nd Extracellular Loop, AA 153-167

Fonction: A Rabbit Polyclonal Antibody to Human Free Fatty Acid Receptor 2 (GPR43)

Réactivité croisée (Details): Not recommended for mouse or rat samples.

Homologie: Human only

Attributs du produit: Anti-Human GPR43/FFAR2 (extracellular) Antibody (ABIN7043139, ABIN7044283 and ABIN7044284) is a highly specific antibody directed against an epitope of the human Free fatty acid receptor 2. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize FFAR2 from human samples only. It will not recognize the protein from rat or mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)TEQVRSGNEIT(S)YEN, corresponding to amino acid residues 153-167 of human FFAR2

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur FFAR2

Antigène: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Autre désignation: FFAR2

Sujet:

Synonyms: G-protein coupled receptor 43, Free fatty acid receptor 2

Description: Free fatty acids (FFAs) are essential nutrients that are also involved in signal transduction through FFA receptors, members of the G-protein coupled receptor superfamily.FFAR2/GPR43 is an FFA receptor that is activated specifically by short fatty acids (acetate, propionate and butyrate), that are the products of bacterial fermentation in the gut1.GPR43 is expressed in adipose tissue, intestines, and immune tissues2 and its stimulation by interaction with short fatty acids inhibits cAMP production, activates the extracellular signal-regulated kinase (ERK) cascade via interactions with the Gi/o family of G-proteins. This increases intracellular Ca2+ levels, and promotes activation of the mitogen-activated protein kinase (MAPK) cascade through interactions with the Gq family of G-proteins1.These processes, mediated by GPR43 receptor regulate the host energy homeostasis and hence GPR43 is shown to be related to metabolic disorders, such as obesity and diabetes1.Because of its pivotal role in energy homeostasis and in regulating many cellular processes, GPR43 has many phenotypic effects: mutant mice are hyper susceptible to developing intestinal carcinogenesis3, Klebsiella pneumoniae infection4 and to increased differentiation of bone marrow cells into osteoclasts5. Studies have shown the role of GPR43 in regulating inflammatory responses, which can lead to colitis and asthma6,7. The various activities mediated by GPR43 makes it an appealing therapeutic target.

ID gène: 2867

NCBI Accession: NM_005306

UniProt: O15552