G Protein-Coupled Receptor 132 anticorps (2nd Extracellular Loop)

N° du produit ABIN7043148

Cet anticorps anti-G Protein-Coupled Receptor 132 Polyclonal Lapin (ABIN7043148) détecte spécifiquement G Protein-Coupled Receptor 132 dans WB, FACS et LCI. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour G Protein-Coupled Receptor 132 anticorps (2nd Extracellular Loop) (ABIN7043148)

Antigène: G Protein-Coupled Receptor 132 (GPR132)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp G Protein-Coupled Receptor 132 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-G Protein-Coupled Receptor 132 anticorps

Épitope: 2nd Extracellular Loop, AA 181-195

Fonction: A Rabbit Polyclonal Antibody to GPR132

Réactivité croisée (Details): The antibody will not recognize rat or mouse samples.

Homologie: Human only

Attributs du produit: Anti-Human GPR132/G2A (extracellular) Antibody (ABIN7043148, ABIN7044431 and ABIN7044432) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR132 from human samples only. The antibody will not recognize GPR132 from mouse and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)EDKET(S)FDMLQMDSR, corresponding to amino acid residues 181 - 195 of human GPR132

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur G Protein-Coupled Receptor 132

Antigène: G Protein-Coupled Receptor 132 (GPR132)

Autre désignation: GPR132

Sujet:

Synonyms: G protein-coupled receptor 132, G2 accumulation protein, G2A

Description: G-protein coupled receptor 132, (GPR132, G2A) is classified as a member of the proton sensing G-protein coupled receptor (GPCR) subfamily. Like other members of this subfamily, i.e. GPR4, OGR1 (GPR68), and TDAG8 (GPR65), G2A is expressed on the cell surface membrane and contains seven transmembrane domains, similar to serpentine receptors. GPR132 senses changes in extracellular pH , and activation of this proton-sensing receptor by acidosis transduces multiple downstream G protein signaling pathways1.GPR132 is expressed in a broad range of immunoregulatory cell types, including macrophages, dendritic cells, neutrophils, mast cells, T and B lymphocytes. It mediates immune cell migration as well as phagocytosis and regulates autoimmunity and lymphocyte homeostasis2.G2A also plays a role in tumor suppression: the tumor microenvironment is acidic due to glycolytic cancer cell metabolism, hypoxia, and deficient blood perfusion and hence, G2A and other proton sensing receptors may be involved in regulation of cancer cell metastasis and proliferation. Small molecule modulators of the pH -sensing GPCRs are being actively developed and evaluated. Research on pH -sensing GPCRs provides important insights into the molecular interaction between the tumor and its acidic microenvironment and may identify new targets for cancer therapy3.

ID gène: 29933

NCBI Accession: NM_013345

UniProt: Q9UNW8