GPR34 anticorps (Extracellular)

N° du produit ABIN7043153

L’anticorps Lapin Polyclonal anti-GPR34 a été validé pour WB et FACS. Il convient pour détecter GPR34 dans des échantillons de Souris.

Aperçu rapide pour GPR34 anticorps (Extracellular) (ABIN7043153)

Antigène: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR34 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS)

Détail du produit anti-GPR34 anticorps

Épitope: AA 197-209, Extracellular

Fonction: A Rabbit Polyclonal Antibody to GPR34

Specificité: 2nd extracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,rat - 12,13 amino acid residues identical

Attributs du produit: Anti-GPR34 (extracellular) Antibody (ABIN7043153, ABIN7044449 and ABIN7044450) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235396)

Blocking Peptide: (ABIN7235396)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR34

Antigène: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Autre désignation: GPR34

Sujet: G-protein coupled receptor 34, LYPSR1,The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system-derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies shows that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages, GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients, GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.

Alternative names: G-protein coupled receptor 34

ID gène: 23890

NCBI Accession: NM_005300

UniProt: Q9R1K6