GPR35 anticorps (Extracellular)

N° du produit ABIN7043156

L’anticorps Lapin Polyclonal anti-GPR35 a été validé pour WB, IHC, FACS et LCI. Il convient pour détecter GPR35 dans des échantillons de Souris.

Aperçu rapide pour GPR35 anticorps (Extracellular) (ABIN7043156)

Antigène: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR35 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-GPR35 anticorps

Épitope: AA 156-170, Extracellular

Fonction: A Rabbit Polyclonal Antibody to GPR35 (extracellular)

Specificité: Extracellular, 2nd loop.

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): Not recommended for human samples.

Homologie: Mouse - 14 out of 15 amino acid residues identical, rat - 12 out of 15 amino acid residues identical Not recommended for human samples

Attributs du produit: Anti-GPR35 (extracellular) Antibody (ABIN7043156, ABIN7044441 and ABIN7044442) is a highly specific antibody directed against an extracellular epitope of the mouse GPR35. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR35 from mouse and rat samples. The antibody will not recognize the receptor from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EGGF(S)FSSQTRRNFS, corresponding to amino acid residues 156 - 170 of mouse GPR35

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235400)

Blocking Peptide: (ABIN7235400)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR35

Antigène: GPR35 (G Protein-Coupled Receptor 35 (GPR35))

Autre désignation: GPR35

Sujet: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor,G- protein coupled receptor 35 (GPR35) is an "orphan" GPCR, whose native ligand is still unknown. It was shown to be involved in the modulation of synaptic transmission, nociception, neuropathic and inflammatory pain, and in various diseases including ulcerative colitis and primary sclerosing cholangitis1 and hence there is a great therapeutic potential in studying its activity.In vitro, GPR35 is stimulated by high concentrations of a number of small molecules, including kynurenic acid, 2-oleoyllysophosphatidic acid, DHICA, reverse T3, cGMP and it has a wide range of synthetic agonists and antagonists that either activate or inactivate it, respectively (Summarized in2). GPR35 is composed of seven trans-membrane domains, and it was shown to share structural homology with various receptors for chemokines, chemotactic cytokines that direct the traffic of leukocytes and other cells in the body. It was shown to be activated by chemokine CXCL17, and hence the role of a chemokine receptor was also assigned to it3.GPR35 expression has been identified within discrete regions of the central nervous system (CNS) and peripheral nervous system, and lower levels in heart, lung and skeletal muscle, and also in the lower gut and colon and by various white cell groups, including numerous dendritic cell and monocyte populations4.

Alternative names: G-Protein Coupled Receptor 35, Kynurenic Acid Receptor

ID gène: 64095

NCBI Accession: NM_001195382

UniProt: Q9ES90