GPR52 anticorps (AA 239-253)

N° du produit ABIN7043161

Cet anticorps Lapin Polyclonal détecte spécifiquement GPR52 dans WB, IHC et IF. Il présente une réactivité envers Souris.

Aperçu rapide pour GPR52 anticorps (AA 239-253) (ABIN7043161)

Antigène: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR52 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-GPR52 anticorps

Épitope: AA 239-253

Fonction: A Rabbit Polyclonal Antibody to GPR52

Specificité: 3rd intracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: human - 13,Rat - 14,15 amino acid residues identical

Attributs du produit: Anti-GPR52 Antibody (ABIN7043161, ABIN7044453 and ABIN7044454) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry. It has been designed to recognize GPR52 from human, mouse, and rat samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRRARFPSHEVEASR, corresponding to amino acid residues 239 - 253 of mouse GPR52

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235423)

Blocking Peptide: (ABIN7235423)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR52

Antigène: GPR52 (G Protein-Coupled Receptor 52 (GPR52))

Autre désignation: GPR52

Sujet: G-Protein Coupled Receptor 52,G-Protein Coupled Receptor 52 (GPR52) is a conserved class-A orphan receptor, a member of the of the GPCR superfamily. The native ligand for GPR52 is unknown but the receptor has a high level of basal activity not related to ligand binding. Structure analysis shows that GPR52 extracellular loop 2 occupies the orthosteric binding pocket and operates as a built-in agonist, enabling an intrinsically high level of basal activity. The receptor also features a side pocket for ligand binding3.Tissue distribution analysis of human, mouse, and rat indicates that GPR52 is expressed exclusively in the brain, especially in striatum. In the human brain, GPR52 expression profile overlaps with the distribution of D1 and D2 dopamine receptors which are associated with its function1.GPR52 inhibits dopamine D2 receptor signaling and activates dopamine D1/NMDA receptors via intracellular cAMP accumulation2.GPR52 is involved in the manifestation of schizophrenia, Huntington's disease, cognitive impairment and hyperactivity and several other psychiatric disorders and hence it represents a promising therapeutic target. Agonists may potentially act as a novel class of antipsychotics1,2.

Alternative names: G-Protein Coupled Receptor 52, GPR52

ID gène: 620246

NCBI Accession: NM_005684

UniProt: P0C5J4