Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) anticorps

N° du produit ABIN7043165

L’anticorps anti-Adhesion G Protein-Coupled Receptor G1 Polyclonal Lapin est utilisé pour la détection de Adhesion G Protein-Coupled Receptor G1 dans des échantillons de Rat. Il a été validé pour FACS, WB et LCI.

Aperçu rapide pour Adhesion G Protein-Coupled Receptor G1 (ADGRG1) (AA 86-109), (Extracellular) anticorps (ABIN7043165)

Antigène: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Western Blotting (WB), Live Cell Imaging (LCI)

Détail du produit

Épitope: AA 86-109, Extracellular

Fonction: A Rabbit Polyclonal Antibody to GPR56

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): This antibody recognizes the N-terminal fragment (GPR56-NT), that can be secreted to the media.

Homologie: 14 amino acid residues identical,Mouse - 13, human - 11

Attributs du produit: Anti-GPR56 (extracellular) Antibody (ABIN7043165, ABIN7044433 and ABIN7044434) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect flow cytometry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize ADGRG1 from rat, mouse, and human samples. The antibody recognizes the N-terminal fragment (GPR56-NT) that can be secreted to the media.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RHTGRLHLRYGKND, corresponding to amino acid residues 86-109 of rat GPR56

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235431)

Blocking Peptide: (ABIN7235431)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détail du antigène

Antigène: Adhesion G Protein-Coupled Receptor G1 (ADGRG1)

Autre désignation: ADGRG1

Sujet: G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1,GPR56 (ADGRG1) is a member of the adhesion G-protein coupled receptor (GPCR) family, responsible for the regulation of cell adhesion, migration, polarity, and guidance. GPR56 levels are increased in many cancer types and seems to be relevant for tumor cell migration and invasion1,2. GPR56 structure is characterized by a long extracellular domain that is involved in cell-to-cell and cell-to-extracellular matrix interactions. The receptor contains a GPS motif, responsible for cleaving the protein. Seven different glycosylation sites along its N-terminal fragment are present1-3.Expression of Gpr56 mRNA can be found in neuronal progenitor cells of the cerebral cortical ventricular and subventricular zones during periods of neurogenesis. Studies show that GPR56 protein knockdown promotes mesenchymal differentiation of glioma stem-like initiating cells, accompanied by increased radioresistance in vitro and in vivo1-3.Mutations in GPR56 may cause bilateral frontoparietal polymicrogyria, a cobblestone-like cortical malformation, characterized by overmigrating neurons and the formation of neuronal ectopias on the surface of the brain2,3.

Alternative names: GPR56, G-protein coupled receptor 56, Adhesion G-protein coupled receptor G1, ADGRG1

ID gène: 260326

NCBI Accession: NM_001145770

UniProt: Q8K3V3