GPR81 anticorps (Intracellular)

N° du produit ABIN7043173

Cet anticorps Lapin Polyclonal détecte spécifiquement GPR81 dans WB et IHC. Il présente une réactivité envers Souris.

Aperçu rapide pour GPR81 anticorps (Intracellular) (ABIN7043173)

Antigène: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR81 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-GPR81 anticorps

Épitope: AA 319-332, Intracellular

Fonction: A Rabbit Polyclonal Antibody to GPR81

Specificité: Intracellular, C-terminus

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): The antibody will not recognize human GPR81 (HCAR1).

Homologie: Rat - 9,14 amino acid residues identical

Attributs du produit: Anti-GPR81/HCAR1 Antibody (ABIN7043173, ABIN7044514 and ABIN7044515) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1)

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235445)

Blocking Peptide: (ABIN7235445)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR81

Antigène: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Autre désignation: HCAR1

Sujet: Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1,GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.

Alternative names: Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1

ID gène: 243270

NCBI Accession: NM_032554

UniProt: Q8C131