GPR83 anticorps (Extracellular)

N° du produit ABIN7043174

L’anticorps Lapin Polyclonal anti-GPR83 a été validé pour WB, IHC, FACS et LCI. Il convient pour détecter GPR83 dans des échantillons de Souris.

Aperçu rapide pour GPR83 anticorps (Extracellular) (ABIN7043174)

Antigène: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GPR83 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-GPR83 anticorps

Épitope: AA 59-71, Extracellular

Fonction: A Rabbit Polyclonal Antibody to GPR83

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,rat - identical

Attributs du produit: Anti-GPR83 (extracellular) Antibody (ABIN7043174, ABIN7044445 and ABIN7044446) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:3000

Application Dilutions Western blot wb: 1:400

Commentaires:

Negative Control: (ABIN7235450)

Blocking Peptide: (ABIN7235450)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GPR83

Antigène: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Autre désignation: GPR83

Sujet: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR,GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

Alternative names: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

ID gène: 14608

NCBI Accession: NM_016540

UniProt: P30731