GJA4 anticorps (AA 131-144)

N° du produit ABIN7043200

Cet anticorps Lapin Polyclonal détecte spécifiquement GJA4 dans WB. Il présente une réactivité envers Rat.

Aperçu rapide pour GJA4 anticorps (AA 131-144) (ABIN7043200)

Antigène: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GJA4 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-GJA4 anticorps

Épitope: AA 131-144

Fonction: A Rabbit Polyclonal Antibody to Connexin-37

Specificité: Intracellular loop

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Attributs du produit: Anti-Connexin-37 Antibody is directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (ABIN7043200, ABIN7044081 and ABIN7044082) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Commentaires:

Negative Control: (ABIN7235535)

Blocking Peptide: (ABIN7235535)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur GJA4

Antigène: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Autre désignation: GJA4

Sujet: CX37, Gap junction α4 protein, GJA4,Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2. Connexins do not function as single proteins, six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

Alternative names: Connexin-37, CX37, Gap junction alpha4 protein, GJA4

ID gène: 25655

NCBI Accession: NM_002060

UniProt: Q03190

Pathways: Cell-Cell Junction Organization