GFRA4 anticorps (Extracellular, N-Term)

N° du produit ABIN7043213

L’anticorps anti-GFRA4 Polyclonal Lapin est utilisé pour la détection de GFRA4 dans des échantillons de Rat. Il a été validé pour WB, IHC, IF, IC et LCI.

Aperçu rapide pour GFRA4 anticorps (Extracellular, N-Term) (ABIN7043213)

Antigène: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GFRA4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Détail du produit anti-GFRA4 anticorps

Épitope: AA 160-172, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to GDNF Family Receptor α4

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): Not recommended for human samples. The antibody will recognize both the membrane bound canonical isoform and the putative secreted isoform.

Homologie: mouse - 11,Rat - 12,13 amino acid residues identical

Attributs du produit: Anti-GFR alpha 4 (extracellular) Antibody is directed against an extracellular epitope of rat GDNF receptor α4. Anti-GFR alpha 4 (extracellular) Antibody (ABIN7043213, ABIN7044781 and ABIN7044782) can be used in western blot, immunohistochemistry, immunocytochemistry and live cell imaging applications. It has been designed to recognize GFR α4 from mouse and rat samples. The antibody is not suitable for detecting GFR α4 from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)GSRDG(S)PEEGGPR, corresponding to amino acid residues 160-172 of rat GFRA4

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:800

FC: The optimal concentration should be determined by the user

ICC: 1:100

IHC: 1:100

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235584)

Blocking Peptide: (ABIN7235584)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GFRA4

Antigène: GFRA4 (GDNF Family Receptor alpha 4 (GFRA4))

Autre désignation: GFRA4

Sujet:

Synonyms: GFRA4, GFRα4, GDNF family receptor α4, GDNF receptor alpha 4, Persephin receptor

Description: The GDNF family ligands (GFLs) belong to the super family of the TGF-β. They belong to the group of cystine-knot protein and function as homodimers1. This family includes glial cell line-derived neurotrophic factor (GDNF), artemin (ARTN), Neurturin (NRTN) and persephin (PSPN)2.These factors are heavily involved in the development and function of the nervous system (both central and peripheral). In particular GDNF has an important role outside the nervous system where it plays a role in kidney morphogenesis1.In general GFLs all signal through a signal through the receptor tyrosine kinase Ret. Their specificity is implemented by different GDNF family receptor a (GFRα), which act as co-receptors. These extracellular proteins are bound to the plasma membrane via a glycosyl phosphatidyl inositol (GPI) anchor. GFRα1-4 are responsible for the binding of GDNF, NRTN, ARTN, and PSPN respectively and the subsequent activation of Ret. Soluble forms of the receptor by the cleavage of a yet unknown phospholipase or protease can be detected1,3. Also alternative spliced forms of the protein can lead to soluble forms of GFRα receptors3.

ID gène: 66023

NCBI Accession: NM_145762

UniProt: Q9EPI2