Gephyrin anticorps (Intracellular)

N° du produit ABIN7886511

Cet anticorps anti-Gephyrin Polyclonal Lapin (ABIN7886511) détecte spécifiquement Gephyrin dans WB, IHC et IF. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour Gephyrin anticorps (Intracellular) (ABIN7886511)

Antigène: Gephyrin (GPHN)

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Gephyrin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-Gephyrin anticorps

Épitope: AA 183-195, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Gephyrin

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-Gephyrin Antibody is directed against an epitope of rat Gephyrin. Anti-Gephyrin Antibody (ABIN7043214, ABIN7044520 and ABIN7044521) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPHN from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RDAIVKVKEVHDE, corresponding to amino acid residues 183-195 of rat Gephyrin

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235588)

Blocking Peptide: (ABIN7235588)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Gephyrin

Antigène: Gephyrin (GPHN)

Autre désignation: GPHN

Sujet:

Synonyms: GPHN, Putative glycine receptor-tubulin linker protein, MPT adenylyltransferase, MPT Mo-transferase

Description: Gephyrin is a highly conserved, intracellular scaffolding protein responsible for the post-synaptic anchoring and assembly of the inhibitory glycine and γ-aminobutyric acid type A receptors (GlyR and GABAAR), it essentially anchors the receptors, by auto-oligomerization, to the cytoskeleton1,2.GABAergic transmission is important for the mediation of neuronal excitability and has a crucial role in neurodevelopment, throughout the central and peripheral nervous systems (especially via GABAAR). Glycinergic neurons on the other hand control motor functions and sensory signaling as well as take part in pain transmission in the spine's dorsal horn. Importantly, gephyrin has a 1:1 stoichiometry with GlyR3,4.Up-to-date atomic force microscopy data of gephyrin has shown that it forms trimers with multiple conformations. It is a modular construct with three domains: an N-terminal G-, a central C- and a C-terminal E-domain. Recent work suggests also that gephyrin regulates neuronal differentiation by anchoring small cytoskeletal-related signaling molecules at unique subcellular locations2,5,6.

ID gène: 64845

NCBI Accession: NM_020806

UniProt: Q03555

Pathways: Synaptic Membrane, Skeletal Muscle Fiber Development