GLRA4 anticorps (Extracellular, N-Term)

N° du produit ABIN7043259

Cet anticorps anti-GLRA4 Polyclonal Lapin (ABIN7043259) détecte spécifiquement GLRA4 dans IC, IF, LCI et WB. L’anticorps est réactif avec des échantillons de Souris.

Aperçu rapide pour GLRA4 anticorps (Extracellular, N-Term) (ABIN7043259)

Antigène: GLRA4 (Glycine Receptor, alpha 4 (GLRA4))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp GLRA4 est non-conjugé

Application: Immunochromatography (IC), Immunofluorescence (IF), Live Cell Imaging (LCI), Western Blotting (WB)

Détail du produit anti-GLRA4 anticorps

Épitope: AA 28-42, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to Glycine Receptor α4

Réactivité croisée: Humain, Souris, Rat

Homologie: human - 13,Rat - 14,15 amino acid residues identical

Attributs du produit: Anti-Glycine Receptor alpha4 (extracellular) Antibody is directed against an extracellular epitope of the mouse glycine receptor α4. Anti-Glycine Receptor α4 (extracellular) Antibody (ABIN7043259, ABIN7044404 and ABIN7044405) can be used in western blot and live cell imagine applications. The antibody recognizes an extracellular epitope and thus is ideal for detecting GlyRα4 in living cells. It has been designed to recognize GlyRα4 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KEDVKSGLKGSQPMS, corresponding to amino acid residues 28-42 of mouse GlyRalpha4

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:500

FC: The optimal concentration should be determined by the user

ICC: 1:50

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7235721)

Blocking Peptide: (ABIN7235721)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur GLRA4

Antigène: GLRA4 (Glycine Receptor, alpha 4 (GLRA4))

Autre désignation: GLRA4

Sujet:

Synonyms: Glycine receptor subunit alpha-4, GLRA4

Description: The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyR is a member of the pentameric Cys-loop ion channel receptor family. Functional Cys-loop receptors comprise homomeric or heteromeric pentameric oligomers with each of the five subunits arranged symmetrically in a ring around a central ion-conducting pore. Several developmentally and regionally regulated GlyR isoforms exist, which result from a differential expression of the GlyR α (α1- α4) and β subunit genes. Each GlyR subunit comprises a large extracellular amino-terminal domain that harbors the ligand-binding sites and the eponymous Cys-loop. This connects to a bundle of four α-helical transmembrane domains (labelled M1-M4) with a large intracellular domain between M3 and M4 and a short extracellular C-terminal tail2. The human α4 subunit is considered a pseudogene on the grounds that it incorporates a premature stop codon upstream of the final TM4 domain3.The α4 subunit has been found in the adult mouse retina where it clusters together with synaptic markers such as bassoon or gephyrin. Furthermore, GlyR α4 transcripts have been detected in the white matter tract of the developing spinal cord from mice. The avian α4 mRNA was also found in dorsal root ganglia and genital ridge 4.Although the function of GlyR α4 at its sites of expression is still unknown, homomeric α4 receptors are able to form fully functional glycine-gated Cl- channels in vitro that can be antagonized with strychnine5.

ID gène: 14657

NCBI Accession: NR_164162

UniProt: Q61603