HOMER1 anticorps (Intracellular)

N° du produit ABIN7043273

L’anticorps Lapin Polyclonal anti-HOMER1 a été validé pour WB, IHC et IF. Il convient pour détecter HOMER1 dans des échantillons de Rat.

Aperçu rapide pour HOMER1 anticorps (Intracellular) (ABIN7043273)

Antigène: HOMER1 (Homer Homolog 1 (HOMER1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HOMER1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-HOMER1 anticorps

Épitope: AA 293-296, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Homer1

Specificité: Intracellular

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Homologie: Mouse,human - identical

Attributs du produit: Anti-Homer1 Antibody (ABIN7043273, ABIN7045168 and ABIN7045169) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer1 from human, mouse, and rat samples. The antibody will not recognize Homer1 short isoform 3 (also known as Homer1a), but will recognize isoform 2 (also known as Homer1b).

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KQEIDNARELQEQR, corresponding to amino acid residues 293-296 of rat Homer1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:500

Commentaires:

Negative Control: (ABIN7235762)

Blocking Peptide: (ABIN7235762)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur HOMER1

Antigène: HOMER1 (Homer Homolog 1 (HOMER1))

Autre désignation: HOMER1

Sujet: Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47,Homer proteins regulate signal transduction, generation of synapses and receptor trafficking. The homer protein family is comprised of three members with each member alternatively spliced to long and short forms. All members of the Homer family are synaptic scaffolding proteins with an N-terminal EVH (Ena/VASP homology 1) and a C-terminal coiled-coil domain. Long forms of the protein contain both EVH1 and coiled-coil domains and are constitutively expressed, whereas the short forms contain only the EVH1 domain and are expressed in an activity dependent manner1.The long form of Homer1 assembles group 1 metabotropic glutamate receptors mGluR1 and mGluR5 in large macromolecular complexes at synapses as well as with components of the NMDA receptor complex at the neuronal cell membrane. In contrast, the short form of Homer1 lacks the ability of linking mGluR1/5 to synaptic proteins and functions as an endogenous negative modulator of the mGluR1/5-inositol 1,4,5-trisphosphate receptor signaling complex. Transgenic mice with overexpressed Homer1 in striatal medium spiny neurons demonstrate normal development of striatal structure and afferent/efferent connectivity. However, motor performance in behavioral tasks and striatal responses to the psychomotor stimulant amphetamine are significantly altered in these mice2.At concentrations

Alternative names: Homer1, Homer protein homolog 1, Homer scaffolding protein 1, Vesl-1, Ves 1, PSD-Zip45, SYN47

ID gène: 29546

NCBI Accession: NM_004272

UniProt: Q9Z214

Pathways: Skeletal Muscle Fiber Development