LRRC4B anticorps (Extracellular)

N° du produit ABIN7043300

L’anticorps Lapin Polyclonal anti-LRRC4B a été validé pour WB, IHC et IF. Il convient pour détecter LRRC4B dans des échantillons de Souris.

Aperçu rapide pour LRRC4B anticorps (Extracellular) (ABIN7043300)

Antigène: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LRRC4B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-LRRC4B anticorps

Épitope: AA 498-511, Extracellular

Fonction: A Rabbit Polyclonal Antibody to NGL-3/LRRC4B

Specificité: Extracellular, N-terminus

Réactivité croisée: Souris, Rat

Homologie: Rat - identical, human - 13 out of 14 amino acid residues identical

Attributs du produit: Anti-/LRRC4B (extracellular) Antibody (ABIN7043300, ABIN7044736 and ABIN7044737) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It recognizes an extracellular epitope and can potentially detect the protein in living cells. It has been designed to recognize LRRC4B from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)PGEEAQQPRGTEKE, corresponding to amino acid residues 498-511 of mouse NGL-3

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7235861)

Blocking Peptide: (ABIN7235861)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur LRRC4B

Antigène: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Autre désignation: LRRC4B

Sujet: Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4,NGL-3 (Netrin G ligand 3) is a postsynaptic cell adhesion molecule that is a member of the NGL family. NGLs are proteins involved in the regulation of various steps in synapse formation. Members of this family share a common domain structure that contains: nine Leucine rich repeats (LRRs), an immunoglobulin (Ig) domain in the extracellular region, a single trans membrane domain and a cytoplasmic region that ends with a PDZ domain-binding motif. This motif mediates the interaction with PSD-95, an abundant postsynaptic scaffolding protein1,2.The NGL family is comprised of three proteins: NGL-1, 2, and 3. NGL-1 and NGL-2 interact through their extracellular domain with the GPI anchored membrane proteins Netrin G1 and G2 respectively2-4. This interaction is important for regulation of structural and functional excitatory synapse development4.NGL-3 trans-synaptically interacts with the leukocyte common antigen-related (LAR) protein which is well-known for its involvement in axon guidance and presynaptic differentiation. This interaction induces pre- and postsynaptic differentiation in contacting axons and dendrites, respectively, and regulates excitatory synapse formation2,5.Mice lacking NGL-3 (Ngl3-/-) show markedly suppressed normal brain development and postnatal survival and growth6. NGL-3 overexpression and knockdown in cultured neurons result in bidirectional changes in the number of excitatory synapses and spontaneous excitatory synaptic transmission5.NGL-3 mRNA and proteins are mainly detected in synaptic fractions of the brain and levels gradually increase during the first 3 weeks of postnatal rat brain development6.

Alternative names: NGL-3/LRRC4B, Netrin-G ligand 3, Netrin-G3 ligand, Leucine-rich repeat-containing protein 4B, LRIG4

ID gène: 272381

NCBI Accession: NM_001080457

UniProt: P0C192

Pathways: Synaptic Membrane