Leukotriene B4 Receptor/BLT anticorps (2nd Extracellular Loop)

N° du produit ABIN7043307

Cet anticorps anti-Leukotriene B4 Receptor/BLT Polyclonal Lapin (ABIN7043307) détecte spécifiquement Leukotriene B4 Receptor/BLT dans FACS et WB. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Leukotriene B4 Receptor/BLT anticorps (2nd Extracellular Loop) (ABIN7043307)

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Leukotriene B4 Receptor/BLT est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB)

Détail du produit anti-Leukotriene B4 Receptor/BLT anticorps

Épitope: 2nd Extracellular Loop, AA 168-181

Fonction: A Rabbit Polyclonal Antibody to BLT1

Réactivité croisée (Details): Not recommended for use with rat and mouse samples.

Homologie: Monkey - identical

Attributs du produit: Anti-Human BLT1 (extracellular) Antibody (ABIN7043307 and ABIN7044557) is a highly specific antibody directed against an extracellular epitope of the human Leukotriene B4 receptor 1. The antibody can be used for western blot and indirect flow cytometry applications. It has been designed to recognize LTB4R from human samples only.

Purification: Affinity purified on immobilized antigen.

Immunogène: CFPRYPSEGHRAFH, corresponding to amino acid residues 168-181 of human LTB4R

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 1:25

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Leukotriene B4 Receptor/BLT

Antigène: Leukotriene B4 Receptor/BLT (LTB4R) (Leukotriene B4 Receptor (LTB4R))

Autre désignation: LTB4R

Sujet:

Synonyms: Leukotriene B4 receptor 1, LTB4R, LTB4R1, CMKRL1, GPR16, P2Y7, P2RY7

Description: Leukotrienes (LTs) are lipid mediators prominently exerting proinflammatory responses. They are divided in two classes: cysteinyl LTs (cysLTs) which have a thioester linkage and LTB41. LTB4, a chemoattractant, plays a prominent role in the recruitment and activation of leukocytes2.LTB4 acts through two receptors which belong to the G-protein coupled receptor superfamily: BLT1 and BLT2. BLT1 displays high affinity for LTB4 and is highly expressed in leukocytes including granulocytes, macrophages, mast cells, dendritic cells and effector T cells3 and is expressed to much lower levels in the spleen, thymus, heart, skeletal muscle, brain and liver2-4. BLT2 on the other hand is a low affinity receptor which does not display selectivity towards LTB45. Its expression is much more ubiquitous than that of BLT1. It is expressed in most human tissues, with the highest expression being in the spleen, liver, ovary, and leukocytes2,5-8. The two receptors could couple and activate different G-proteins, depending on the cell type and the cellular events activating the receptors2. However, activation of BLT1 usually correlates with increasing intracellular concentrations of Ca2+.The LTB4/BLT1 system is involved in many allergic reactions as well as asthma induced as an allergic response, where it plays a significant role in recruiting neutrophils and effector T cells into lungs as part of an inflammatory response induced by allergens1.

ID gène: 1241

NCBI Accession: NM_001143919

UniProt: Q15722