LPAR1 anticorps (Extracellular)

N° du produit ABIN7043311

Cet anticorps Lapin Polyclonal détecte spécifiquement LPAR1 dans WB, IHC, FACS et LCI. Il présente une réactivité envers Rat.

Aperçu rapide pour LPAR1 anticorps (Extracellular) (ABIN7043311)

Antigène: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LPAR1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Détail du produit anti-LPAR1 anticorps

Épitope: AA 36-50, Extracellular

Fonction: A Rabbit Polyclonal Antibody to LPA Receptor 1 (EDG2)

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Attributs du produit: Anti-LPAR1 (EDG2) (extracellular) Antibody (ABIN7043311, ABIN7044566 and ABIN7044567) is a highly specific antibody directed against an epitope of the rat LPA1 receptor. The antibody can be used in western blot analysis. It has been designed against an extracellular domain and thus is highly suitable for detecting the receptor in living cells. It has been designed to recognize LPA1 receptor from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSGKYLATEWNTVSK, corresponding to amino acid residues 36-50 of rat LPA1 receptor

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:400

Commentaires:

Negative Control: (ABIN7235898)

Blocking Peptide: (ABIN7235898)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur LPAR1

Antigène: LPAR1 (Lysophosphatidic Acid Receptor 1 (LPAR1))

Autre désignation: LPAR1

Sujet: Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1,Lysophosphatidic acid is a naturally occurring lysophospholipid that activates diverse cellular actions in various cells including proliferation and morphological changes. There are currently three known genes that encode proteins of the LPA receptor family- Edg2, Edg4 and Edg7.Lysophosphatidic acid receptor 1 (also known as Lpa1), encoded by Edg2, belongs to G-coupled protein receptor (GPCR) superfamily. All three proteins share 50-54 % identical amino-acids. Lpa1 mostly activates the Gi pathway that leads to inhibition of adenylate cyclase and thus inhibition of cAMP production. Through this pathway, Lpa1 also initiates the Ras/MAP kinase cascade which is primarily responsible for increased cell proliferation1. Interestingly, binding of lysophosphatidic acid to the G13 G-protein activates the small GTP-binding protein Rho and induces the rapid remodeling of the actin cytoskeleton2.Lpa1 has been implicated in the pathogenesis of metastatic bone cancer. Silencing of Lpa1 expression in breast and ovarian cancer cells significantly alters the progression of bone metastasis through reduction of tumor burden and inhibition of bone resorption. A specific Lpa1 antagonist exhibits specificity for tumor cell-platelet interaction without abrogating normal platelet function3.

Alternative names: LPAR1 (EDG2), Lysophosphatidic acid receptor 1, LPA receptor 1, LPA-1, Gpcr91, Gpr26, VZG1

ID gène: 116744

NCBI Accession: NM_057159

Pathways: Myometrial Relaxation and Contraction, Smooth Muscle Cell Migration