MCHR1 anticorps (Extracellular)

N° du produit ABIN7043320

Cet anticorps Lapin Polyclonal détecte spécifiquement MCHR1 dans WB, IF, IC et LCI. Il présente une réactivité envers Rat.

Aperçu rapide pour MCHR1 anticorps (Extracellular) (ABIN7043320)

Antigène: MCHR1 (Melanin-Concentrating Hormone Receptor 1 (MCHR1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCHR1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Détail du produit anti-MCHR1 anticorps

Épitope: AA 7-20, Extracellular

Fonction: A Rabbit Polyclonal Antibody to Melanin-Concentrating Hormone Receptor 1

Specificité: Extracellular, N-terminus

Réactivité croisée: Humain, Souris, Rat

Homologie: human - 13,Mouse - 16,17 amino acid residues identical

Attributs du produit: Anti-MCHR1 (extracellular) Antibody (ABIN7043320 and ABIN7044603) is a highly specific antibody directed against an epitope of the rat Melanin-concentrating hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize MCHR1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: CMDLQTSLLSTGPNASN, corresponding to amino acid residues 7-20 of rat MCHR1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Commentaires:

Cited Application: IHC

Negative Control: (ABIN7235912)

Blocking Peptide: (ABIN7235912)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur MCHR1

Antigène: MCHR1 (Melanin-Concentrating Hormone Receptor 1 (MCHR1))

Autre désignation: MCHR1

Sujet: Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1,Melanin-concentrating hormone (MCH) is a 19 amino acid peptide originally isolated from the salmon pituitary gland (in fish the length of the peptide is 17 amino acids)1. In rodents and humans the peptide is synthesized in the brain and is involved in a number of physiological effects, although it is best known for its central role in the control of appetite control. For example, central administration of MCH in mice leads to obesity and conversely, knockout of the MCH gene in mice yields lean animals with an increased metabolic rate and reduced body fat2,3.MCH exerts its effects by binding MCHR1 and MCHR2, two receptors belonging to the superfamily of G-protein coupled receptors (GPCRs). Like all members of the family, MCHR1 and MCHR2 have seven transmembrane domains, an extracellular N-terminal and intracellular C-terminal tails. MCHR1 and MCHR2 activation both lead to an increase in intracellular Ca2+ concentration3.In rat, MCHR1 expression is quite broad, and is strongly detected in the brain. Expression of the receptor is also seen in the eye, skeletal muscle, tongue, pituitary, intestine and lymphocytes4,5. MCHR1 is also expressed in adipose tissue where it regulates leptin secretion3. In human, MCHR1 is mostly localized to the brain and pituitary. MCHR2 displays similar localization to that of MCHR1 in the brain5,6. As MCHR2 is a pseudogene in rodents, studying its function is quite challenging. In the brain, MCHR1 is localized in many areas that are known to be involved in various psychological disorders. Indeed, MCHR1-/- animals display antianxiety- and antidepressant-like behavior7,8. Therefore, antagonizing the activity of MCHR1 could indeed serve as a means to treat depression and obesity9.

Alternative names: MCHR1, Melanin-concentrating hormone receptor 1, MCH1, MCH1R, GPR24, Somatostatin receptor-like protein, SLC-1

ID gène: 83567

NCBI Accession: NM_005297

UniProt: P97639

Pathways: Feeding Behaviour