Melatonin Receptor 1B anticorps (3rd Intracellular Loop)

N° du produit ABIN7043327

L’anticorps anti-Melatonin Receptor 1B Polyclonal Lapin est utilisé pour la détection de Melatonin Receptor 1B dans des échantillons de Souris. Il a été validé pour WB, IHC, IF et IC.

Aperçu rapide pour Melatonin Receptor 1B anticorps (3rd Intracellular Loop) (ABIN7043327)

Antigène: Melatonin Receptor 1B (MTNR1B)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Melatonin Receptor 1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-Melatonin Receptor 1B anticorps

Épitope: 3rd Intracellular Loop, AA 232-246

Fonction: A Rabbit Polyclonal Antibody to Melatonin Receptor Type 1B

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): Unlikely to recognize human samples.

Homologie: Rat - 14,15 amino acid residues identical

Attributs du produit: Anti-Melatonin Receptor 1B (MTNR1B) Antibody (ABIN7043327, ABIN7044601 and ABIN7044602) is a highly specific antibody directed against an epitope of mouse MT2. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MT2 from rat and mouse samples. The antibody is unlikely to recognize the receptor in human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)RKAKATRKLRLRPSD, corresponding to amino acid residues 232-246 of mouse MTNR1B

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:600-1:2000

IP: The optimal concentration should be determined by the user

Commentaires:

Cited Application: IHC|ICC

Negative Control: (ABIN7235940)

Blocking Peptide: (ABIN7235940)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Melatonin Receptor 1B

Antigène: Melatonin Receptor 1B (MTNR1B)

Autre désignation: MTNR1B

Sujet:

Synonyms: Melatonin receptor type 2, MT2, Mel1b receptor, Mel-1B-R

Description: Melatonin (N-acetyl-5-methoxytryptamine) is a product of tryptophan metabolism. It is synthesized in the pineal gland and is secreted to control the circadian rhythm1-3. Melatonin is also synthesized in the gastrointestinal tract, retina, skin and other tissues where it acts in a autocrine or paracrine manner. The role of melatonin in these tissues is independent of its role in the circadian rhythm, where it plays a role in energy metabolism, physiological growth, differentiation and responsiveness in stress stimuli1. The pleiotropic effects of melatonin have given rise to various therapeutic possibilities for this molecule. For example, anti-stress, sexual dysfunction, obesity, gallbladder stones1,4. To date, the only therapeutic uses for melatonin remain to treat sleep disorders, depression, migraine and headaches1.Melatonin exerts its effects through two G-protein coupled receptors (GPCRs), melatonin receptor type 1 and melatonin receptor type 2 (MT1 and MT2). Like all GPCRs, they have seven transmembrane domains and extracellular N-terminal and cytoplasmic C-terminal tails. The binding of melatonin to either receptor activates Gi, thereby activating PLC, thus increasing intracellular Ca2+ levels. Both receptors structurally bind melatonin in the same manner, although MT2 displays a much higher affinity for the hormone. Just like melatonin levels are detected in many tissues, the expression patterns of the two receptors are also quite broad. For example, MT1 is detected in the brain, retina and kidneys and MT2 is expressed in brain and in the retina1.MT1 is involved in sleep regulation and might also have effects on peripheral vasoconstriction. MT2 may play an important physiological role in the retina and might regulate body temperature1.

ID gène: 244701

NCBI Accession: NM_005959

UniProt: Q8CIQ6