MCOLN1 anticorps (Cytoplasmic Domain, Intracellular)

N° du produit ABIN7043336

L’anticorps anti-MCOLN1 Polyclonal Lapin est utilisé pour la détection de MCOLN1 dans des échantillons de Souris. Il a été validé pour WB et IHC.

Aperçu rapide pour MCOLN1 anticorps (Cytoplasmic Domain, Intracellular) (ABIN7043336)

Antigène: MCOLN1 (Mucolipin 1 (MCOLN1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MCOLN1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Classe de qualité: KO Validated

Détail du produit anti-MCOLN1 anticorps

Épitope: AA 6-19, Cytoplasmic Domain, Intracellular

Fonction: A Rabbit Polyclonal Antibody to TRPML1 (Mucolipin 1) Channel

Réactivité croisée: Humain, Souris, Rat

Homologie: Human,rat - 12,14 amino acid residues identical

Attributs du produit: Anti-TRPML1 (Mucolipin 1) Antibody is directed against an epitope of mouse TRPML1. Anti-TRPML1 (Mucolipin 1) Antibody (ABIN7043336, ABIN7044039 and ABIN7044040) can be used in western blot analysis. It has been designed to recognize TRPML1 from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)GRRASETERLLTPN, corresponding to amino acid residues 6-19 of mouse TRPLM1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Commentaires:

Cited Application: IP|ICC

Negative Control: (ABIN7235971)

Blocking Peptide: (ABIN7235971)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur MCOLN1

Antigène: MCOLN1 (Mucolipin 1 (MCOLN1))

Autre désignation: MCOLN1

Sujet:

Synonyms: Mcoln1, Mucolipidin

Description: The endolysosome system takes part in important cellular functions such as membrane trafficking, protein transport, autophagy and signal transduction1. Endosomes result from endocytosis of the plasma membrane and lysosomes (which are derived from late endosomes) conatin mainly hydrolytic enzymes and generally have a low internal pH 1. Like the endoplasmic reticulum (ER), endolysosomes also store Ca2+ (luminal Ca2+ concentration: 0.5 mM)1,2, and similarly to Ca2+ release from the ER, Ca2+ from endolysosomes may also play an important role in various signaling events. To date such candidates include members of the TRP super-family of ion channels and the two-pore Ca2+ channels (TPCs)1,3,4.TRPMLs, also termed mucolipins, are members of the TRP channels. In mammals, three TRPMLs are known to date (TRPML1-3 or MCOLN1-3). They are all localized to endolysosomes, although when over expressed in heterologous systems, TRPML3 is found on the plasma membrane1,5. These channels are Ca2+ permeable and display inward rectifying current properties1,5. Like all members of this family, TRPMLs have six transmembrane domains and intracellular N- and C-termini (relatively short tails compared to other members). They are characterized by an exceptionally large extracellular (luminal) loop between transmembrane domains 1 and 2, and N-glycosylation sites are present in the first extracellular (luminal) loop5.In mammals, TRPML1 is expressed in a ubiquitous manner and shows highest expression in the brain, kidney, spleen, liver and heart1,6. TRPML2 and TRPML3 are less widely expressed. Interestingly, in mouse, two splice variants exist for TRPML2. The shorter variant is more broadly expressed and is dominant over the longer variant in the thymus, spleen and kidney1,7. TRPML3 is highly detected in the thymus, lung, kidney, spleen and eye1,7,8, some epithelial cells1,9 and brain10.Pathologies related to these channels include type IV mucolipidosis, a neurodegenetative disease characterized by retardation and retinal degeneration caused by a loss of function mutation in the gene encoding TRPML1. In contrast, a gain of function mutation in TRPML3, in mice, causes deafness, and pigmentation defects11.

ID gène: 94178

NCBI Accession: NM_020533

UniProt: Q99J21

Pathways: Transition Metal Ion Homeostasis