NCS1 anticorps (Intracellular)

N° du produit ABIN7043367

Cet anticorps anti-NCS1 est un anticorps Lapin Polyclonal détectant NCS1 dans WB et IHC. Adapté pour Rat.

Aperçu rapide pour NCS1 anticorps (Intracellular) (ABIN7043367)

Antigène: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NCS1 anticorps

Épitope: AA 40-53, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1

Specificité: Intracellular

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-NCS1 Antibody is directed against an epitope of the rat Neuronal calcium sensor 1. Anti-NCS1 Antibody (ABIN7043367, ABIN7044204 and ABIN7044205) can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200-1:400

Commentaires:

Negative Control: (ABIN7236071)

Blocking Peptide: (ABIN7236071)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur NCS1

Antigène: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Autre désignation: NCS1

Sujet: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP,Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

Alternative names: NCS1, Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

ID gène: 65153

NCBI Accession: NM_014286

UniProt: P62168