NCS1 anticorps (Intracellular)

N° du produit ABIN7886265

Cet anticorps anti-NCS1 Polyclonal Lapin (ABIN7886265) détecte spécifiquement NCS1 dans WB et IHC. L’anticorps est réactif avec des échantillons de Rat.

Aperçu rapide pour NCS1 anticorps (Intracellular) (ABIN7886265)

Antigène: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NCS1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Détail du produit anti-NCS1 anticorps

Épitope: AA 40-53, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1

Réactivité croisée: Humain, Souris, Rat

Homologie: Mouse,human - identical

Attributs du produit: Anti-NCS1 Antibody is directed against an epitope of the rat Neuronal calcium sensor 1. Anti-NCS1 Antibody (ABIN7043367, ABIN7044204 and ABIN7044205) can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Commentaires:

Negative Control: (ABIN7236071)

Blocking Peptide: (ABIN7236071)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur NCS1

Antigène: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Autre désignation: NCS1

Sujet:

Synonyms: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

Description: Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

ID gène: 65153

NCBI Accession: NM_014286

UniProt: P62168