NETO1 anticorps (Extracellular)

N° du produit ABIN7043376

Cet anticorps anti-NETO1 est un anticorps Lapin Polyclonal détectant NETO1 dans WB, IHC et IF. Adapté pour Souris.

Aperçu rapide pour NETO1 anticorps (Extracellular) (ABIN7043376)

Antigène: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp NETO1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-NETO1 anticorps

Épitope: AA 24-37, Extracellular

Fonction: A Rabbit Polyclonal Antibody to NETO1

Specificité: Extracellular, N-terminus

Réactivité croisée: Souris, Rat

Homologie: Rat - identical, human - 12,14 amino acid residues identical

Attributs du produit: Anti-NETO1 (extracellular) Antibody (ABIN7043376, ABIN7044384 and ABIN7044385) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize NETO1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KKGTEKQITPETQK, corresponding to amino acid residues 24-37 of mouse NETO1

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Commentaires:

Negative Control: (ABIN7236106)

Blocking Peptide: (ABIN7236106)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur NETO1

Antigène: NETO1 (Neuropilin (NRP) and Tolloid (TLL)-Like 1 (NETO1))

Autre désignation: NETO1

Sujet: Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1,NETO proteins are auxiliary subunits that play crucial roles in modulating the biophysical properties of Kainate receptors (KARs) and N-methyl-D-aspartate receptors (NMDARs). NETO proteins modulate these receptors both in vivo and in vitro. NETO1 is also responsible for the slow kinetics of postsynaptic KAR-mediated currents and regulate synaptic recruitment of Kainate receptors1.NETO1 is a single transmembrane domain protein containing two extracellular CUB domains of about 110 amino acids, a low-density lipoprotein receptor domain, and a cytoplasmic tail of almost 167 amino acids that includes a C-terminal TRV class I PDZ binding motif2.NETO1 and NETO2, are highly homologous, but have a different expression patterns and distinct functional effects. NETO1 subunit is expressed in high levels in the developing hippocampus and regulates axonal and presynaptic Kainate receptors at the CA3-CA1 region of the hippocampus. NETO1 deficiency resulted in impairs synaptogenesis and disrupts synchronization of the CA3-CA1 neuronal populations1,3. NETO1 is enriched in the post-synaptic density where it co-localizes with post-synaptic density-95 (PSD-95) and GluN12.

Alternative names: NETO1, Neuropilin and tolloid-like protein 1, Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1, BCTL1, BTCL1

ID gène: 246317

NCBI Accession: NM_138999

UniProt: Q8R4I7

Pathways: Regulation of long-term Neuronal Synaptic Plasticity