Plexin A3 anticorps (Extracellular, N-Term)

N° du produit ABIN7043438

L’anticorps anti-Plexin A3 Polyclonal Lapin est utilisé pour la détection de Plexin A3 dans des échantillons de Souris. Il a été validé pour IHC, WB, FACS, IF et LCI.

Aperçu rapide pour Plexin A3 anticorps (Extracellular, N-Term) (ABIN7043438)

Antigène: Plexin A3 (PLXNA3)

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Plexin A3 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Live Cell Imaging (LCI)

Détail du produit anti-Plexin A3 anticorps

Épitope: AA 440-453, Extracellular, N-Term

Fonction: A Rabbit Polyclonal Antibody to Plexin-A3

Réactivité croisée: Humain, Souris, Rat

Homologie: Rat - identical, human - 12,14 amino acid residues identical

Attributs du produit: Anti-Plexin-A3 (extracellular) Antibody (ABIN7043438, ABIN7045134 and ABIN7045135) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and indirect live cell flow cytometry. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize PLXNA3 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)KVRVDGSQDAQLYE, corresponding to amino acid residues 440-453 of mouse PLXNA3

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Cited Application: IHC

Negative Control: (ABIN7236268)

Blocking Peptide: (ABIN7236268)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Plexin A3

Antigène: Plexin A3 (PLXNA3)

Autre désignation: PLXNA3

Sujet:

Synonyms: PLXNA3, PLXN3, Semaphorin receptor SEX

Description: Plexins are type 1 transmembrane (TM) receptors acting as key regulatory proteins in a wide variety of developmental, regenerative and pathological processes. They are involved in guidance and motility of vascular, lymphatic vessel, and neuron growth, and in cancer metastasis.Plexin family includes 9 proteins: Plexin-A1-4, -B1-3, -C1 and -D1. Class A Plexins interact with the secreted class 3 Semaphorins, and require Neuropilins (nrps) as co-receptors.Plexin protein signaling results in cytosolic GTPase-activating protein activity including direct interactions with Rho GTPases and transient/catalytic interactions with Ras GTPases1,2.Plexin structure consists of an extracellular semaphorin domain, three Plexin-Semaphorin-Integrin (PSI) domains, and three immunoglobulin, Plexin, and transcription factor (IPT) domains. In addition, a single-spanning transmembrane domain, and a cytosolic region (CYTO) homologous with Ras GTPase-activating proteins (GAPs)2. The transmembrane region of Plexin-A3 is characterized by poly-glycine usually used for close helix-helix packing and aromatic residues that distort helix conformation and association. Plexin-A family members only have 22 residues spanning the membrane1.Mutations in Plexins have been reported in melanoma, lung, breast, pancreatic, and prostate cancers, strongly suggesting that their signaling may play a role in cancer development. Plexins are widely expressed in the nervous system and in the developing cerebral cortex2.

ID gène: 18846

NCBI Accession: NM_017514

UniProt: P70208

Pathways: Regulation of Cell Size