KCNJ3 anticorps (Extracellular Loop)

N° du produit ABIN7044555

L’anticorps anti-KCNJ3 Monoclonal Souris est utilisé pour la détection de KCNJ3 dans des échantillons de Humain. Il a été validé pour WB, IF, IC et LCI.

Aperçu rapide pour KCNJ3 anticorps (Extracellular Loop) (ABIN7044555)

Antigène: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp KCNJ3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Clone: 3C3

Détail du produit anti-KCNJ3 anticorps

Épitope: Extracellular Loop

Fonction: A Mouse Monoclonal Antibody to GIRK1 (Kir3.1) Channel

Réactivité croisée: Humain, Souris

Homologie: Kir3.1 from human,rat,mouse samples

Attributs du produit: Mouse Anti-GIRK1 (Kir3.1) (extracellular) Antibody (ABIN7043483 and ABIN7044555) is a highly specific mouse monoclonal antibody directed against an epitope of the human protein. The antibody can be used in western blot, and immunocytochemistry applications. It has been designed to recognize Kir3.1 from human, rat and mouse samples.

Purification: Affinity purified from cultured hybridoma medium.

Immunogène: Synthetic peptide mapping to the extracellular loop of human GIRK1 

Isotype: IgG1

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: 1:20

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL or 100 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur KCNJ3

Antigène: KCNJ3 (Potassium Inwardly-Rectifying Channel, Subfamily J, Member 3 (KCNJ3))

Autre désignation: KCNJ3

Sujet:

Synonyms: KCNJ3, G protein-activated inward rectifier potassium channel 1

Description: Kir3.1 (or G-protein regulated inward-rectifier K+ channel 1, GIRK1) is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heterotetramers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir3.1 is a member of the Kir3.x subfamily that includes four members (Kir3.1- Kir3.4). The Kir3 family is characterized by the fact that the channels can be activated by neurotransmitters and other factors acting via the activation of G-protein coupled receptors. Binding of the corresponding ligand to the G-protein receptor induces the dissociation of Gα-GTP from the Gβγ dimer. The latter directly binds to Kir3 and activates the channel1,3.In the heart, Kir3.1 co-assembles with Kir3.4 to form the prototypical muscarinic-gated K+ channel KAch current, responsible for slowing the heart rate in response of parasympathetic stimulation2.In the brain, Kir3.1 co-assembles with Kir3.2 and mediates the inhibitory effects of many neurotransmitters including opioid, adrenergic, muscarinic, dopaminergic and γ-aminobutyric acid (GABA)1,3.A peptide toxin originating from the Apis mellifera bee venom, Tertiapin (#STT-250) was shown to be a potent blocker of Kir3.1 containing channels (8.6 nM for the Kir3.1/3.4 combination and 5.4 nM for the Kir3.1/3.2)4,5.

ID gène: 3760

NCBI Accession: NM_002239

UniProt: P48549