Pro BDNF anticorps (Pro-Domain)

N° du produit ABIN7043558

L’anticorps anti-Pro BDNF Polyclonal Lapin est utilisé pour la détection de Pro BDNF dans des échantillons de Humain. Il a été validé pour IHC, IF, WB et IP.

Aperçu rapide pour Pro BDNF anticorps (Pro-Domain) (ABIN7043558)

Antigène: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Pro BDNF est non-conjugé

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-Pro BDNF anticorps

Épitope: AA 72-88, Pro-Domain

Fonction: A Rabbit Polyclonal Antibody to proBDNF

Réactivité croisée: Humain, Souris, Rat

Réactivité croisée (Details): The antibody is specific for proBDNF, it does not crossreact with proNGF, proNT-3 or mature BDNF.

Homologie: Mouse,rat - 16,17 amino acid residues identical

Attributs du produit: Anti-proBDNF Antibody (ABIN7043558, ABIN7044753 and ABIN7044754) is a highly specific antibody directed against the prodomain region of human proBDNF. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize proBDNF from human, mouse, and rat samples. The antibody does not cross react with mature BDNF, pro- and mature NGF or mature .

Purification: Affinity purified on immobilized antigen.

Immunogène: (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200-1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Commentaires:

Positive Control: PCP-B257

Cited Application: IP|ICC

Negative Control: (ABIN7236605)

Blocking Peptide: (ABIN7236605)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur Pro BDNF

Antigène: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Autre désignation: proBDNF

Sujet:

Synonyms: Brain-derived neurotrophic factor precursor

Description: Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer's disease.Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.

ID gène: 627

NCBI Accession: NM_170735

UniProt: P23560