TMEM119 anticorps (Extracellular) (FITC)

N° du produit ABIN7043860

Cet anticorps Lapin Polyclonal détecte spécifiquement TMEM119 dans FACS. Il présente une réactivité envers Souris.

Aperçu rapide pour TMEM119 anticorps (Extracellular) (FITC) (ABIN7043860)

Antigène: TMEM119 (Transmembrane Protein 119 (TMEM119))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TMEM119 est conjugé à/à la FITC

Application: Flow Cytometry (FACS)

Détail du produit anti-TMEM119 anticorps (FITC)

Épitope: AA 53-67, Extracellular

Fonction: A Rabbit Polyclonal Antibody to TMEM119 (extracellular) conjugated to the fluorescent dye FITC

Specificité: Extracellular, N-terminus.

Réactivité croisée: Souris, Rat

Réactivité croisée (Details): This antibody will not recognize human TMEM119

Homologie: Rat - 12 out of 15 amino acid residues identical This antibody will not recognize human TMEM119

Attributs du produit: Anti-TMEM119 (extracellular) Antibody (ABIN7043859, ABIN7044740 and ABIN7044741) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry. It has been designed to recognize TMEM119 from rat and mouse samples. The antibody will not recognize the protein from human samples. \nAnti-TMEM119 (extracellular)-FITC Antibody (ABIN7043859, ABIN7044740 and ABIN7044741)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogène:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GTPAFSPTPERPQPT, corresponding to amino acid residues 53 - 67 of mouse TMEM119

Isotype: IgG

Information d'application

Indications d'application:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Commentaires:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7237517)

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Détails sur TMEM119

Antigène: TMEM119 (Transmembrane Protein 119 (TMEM119))

Autre désignation: TMEM119

Sujet: Transmembrane Protein 119, Osteoblast induction factor, OBIF,Transmembrane protein 119, Tmem119, is a bone formation related factor that belongs to a group of bone morphogenetic proteins (BMPs). It is a class IA single pass transmembrane protein1.Tmem119 promotes the differentiation of myoblasts (embryonic precursors of muscle cells) into osteoblasts and plays a role in numerous BMP signaling pathways1,2.Tmem119 is uniquely expressed in microglia cells and it is therefore used as a microglia-specific homeostatic marker that can assist in differentiating microglia cells from peripherally derived macrophages in the brain3. This marker is very important for diagnosing CNS disease: monocytes/macrophages are key cellular modulators of brain homeostasis. In the healthy brain resident microglia are the predominant macrophage cell population but when the blood-brain barrier is leaking peripheral monocytes/macrophages can infiltrate the brain and participate in CNS disease pathogenesis. Distinguishing these two monocytesmacrophages populations is often challenging and hence the existence of a highly expressed and robust microglia marker such as Tmem119 is very important4.

Alternative names: Transmembrane Protein 119, Osteoblast induction factor, OBIF

ID gène: 231633

NCBI Accession: NM_181724

UniProt: Q8R138