Recombinant MUC1 anticorps

N° du produit ABIN7072292

L’anticorps Lapin Chimeric anti-MUC1 a été validé pour IHC, WB, ELISA, IP et IRMA. Il convient pour détecter MUC1 dans des échantillons de Humain.

Aperçu rapide pour Recombinant MUC1 anticorps (ABIN7072292)

Antigène: MUC1 (Mucin 1 (MUC1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MUC1 est non-conjugé

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunoradiometric Assay (IRMA)

Clone: Mc5

Détail du produit anti-MUC1 anticorps

Fonction: Anti-MUC1 [Mc5], Rabbit IgG, kappa

Specificité: This antibody is specific for the amino acid sequence DTRPAP of MUC1. The alpha subunit of MUC1 has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit of MUC-1 contains a C-terminal domain which is involved in cell signaling, through phosphorylations and protein-protein interactions. Modulates signaling in ERK, SRC and NF-kappa-B pathways. In activated T-cells, influences directly or indirectly the Ras/MAPK pathway. Promotes tumor progression. Regulates TP53-mediated transcription and determines cell fate in the genotoxic stress response. Binds, together with KLF4, the PE21 promoter element of TP53 and represses TP53 activity.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2

Purification: Protein A affinity purified

Immunogène: Human breast epithelial mucin.

Isotype: IgG kappa

Information d'application

Indications d'application: In an attempt to develop a reliable way to detect breast cancer, immunoprecipitation was preformed on NPGP from HMFG using the mouse version of this antibody bound to Sepharose 4B. Furthermore, this antibody was tested in an radioimunnology experiment on NPGP using the mouse version of this antibody (Ceriani et al, 1992, pmid:1377884). The epitope of this antibody was mapped by ELISA using small peptides - the murine version of this antibody was used (Petrakou et al, 1998 , pmid:9422085). This antibody was used to identify cells expressing the MUC1 protein. This was accomplished by using the mouse version of this antibody on cell lysates of cell transfected with the MUC1 protein in a western blot analysis (Rubinstein et al, 2006, pmid:17145869). To compare the diagnostic usefulness of different antibodies, the mouse version of this antibody was used for immunohistochemistry on cell pellets from pleural fluid specimens (Creaney et al, 2008, pmid:18454162). While developing an antibody against the alpha/beta junction of MUC1 the mouse version of this antibody was used for western blot on the MUC1 protein (Rubinstein et al, 2008, pmid:18821582). While assesing a panel of 56 anti MUC1 antibodies for their ability to detect cancer the mouse version of this antibody was used for an immunoradiometric assay on a serum made from sera from 10 different patients with breast, ovarian or colon cancer (Norum et al, 1998, pmid:9422099). To more accurately determine the epitope of this antibody, an ELISA was preformed using the murine version of this antibody on different small pepetides from the MUC1 protein (Karsten et al, 2004, pmid:15115750).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MUC1

Antigène: MUC1 (Mucin 1 (MUC1))

Autre désignation: MUC1

Sujet: CD227, BEM, Breast epithelial mucin, Mucin-1, Breast carcinoma-associated antigen DF3, Cancer antigen 15-3, CA 15-3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, KL-6, PEMT, Peanut-reactive urinary mucin, PUM, Polymorphic epithelial mucin, PEM, Tumor-associated epithelial membrane antigen, EMA, Tumor-associated mucin

UniProt: P15941

Pathways: Negative Regulation of intrinsic apoptotic Signaling