Recombinant Fibrin beta-Chain anticorps

N° du produit ABIN7072406

L’anticorps anti-Fibrin beta-Chain Monoclonal Souris est utilisé pour la détection de Fibrin beta-Chain dans des échantillons de Humain. Il a été validé pour ELISA, BI et in vivo.

Aperçu rapide pour Recombinant Fibrin beta-Chain anticorps (ABIN7072406)

Antigène: Fibrin beta-Chain

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: ELISA, BioImaging (BI), In vivo Studies (in vivo)

Clone: 8E5

Détail du produit

Fonction: Anti-Fibrin beta-chain [8E5], Mouse IgG1, kappa

Specificité: 8E5 binds to the amino-terminal region of the β-chain of human fibrin at an epitope consiting of GHRPLDKC. Binding is possible in both the monomeric form and the polymeric form of the protein. The epitope recognised by this clone is only accessible upon conversion of fibrinogen to fibrin, which entails the proteolytic removal of fibrinopeptides A & B from fibrinogen by thrombin. The antibody is there is therefore fibrin specific. Fibrin is a non-globular protein which plays a role in the clotting of blood and circulates as the precursor fibrinogen. Polymerization occurs through the action of the protease thrombin, which results in the active fibrin. Fibrin polymerizes as an insoluble matrix and forms a clot along with platelets over a wound site.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: 8E5 was prepared fusing Sp2/o myeloma cells with spleen cells derived from female BALB/c mice immunized with a synthetic beta-fibrin peptide seqeunce GHRPLDKC conjugated to MB-KLH (maleimidobenzoylated keyhole limpet hemocyanin).

Isotype: IgG1 kappa

Information d'application

Indications d'application: The recombinant scFv version of 8E5 retains the same antigen-binding activity of the parent antibody. 8E5 can bind to fibrin in the monomeric and polymeric form, both in vivo and ex vivo. This antibody can also be used for the immunodetection of fibrin in vivo. Standard ELISA and competitive ELISA using the epitope peptide has been conducted with both the parental antibody and the scFv.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fibrin beta-Chain

Sujet: Factor Ia, factor-1a, fibrin, fibrin β chain, fibrin beta-chain, fibrin beta chain, fibrin β chain, Fibrinopeptide B

UniProt: P02675