Recombinant MHC Class II anticorps

N° du produit ABIN7072422

Cet anticorps anti-MHC Class II est un anticorps Lapin Chimeric détectant MHC Class II dans FACS, IHC et IP. Adapté pour Souris et Rat.

Aperçu rapide pour Recombinant MHC Class II anticorps (ABIN7072422)

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MHC Class II est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: P7-7

Détail du produit anti-MHC Class II anticorps

Fonction: Anti-MHC II [P7/7], Rabbit IgG, kappa

Specificité: This antibody recognises the rat RT1Bu/Du MHC class II antigen, and cross-reacts with haplotypes c, d, l and n. This antibody stains MHC class II molecules in a number of rat strains, including PVG, F334, LEW, and BN. However, this antibody is not reactive with RT1a haplotype rats, including Wistar rats. This antibody also cross-reacts with murine I-A and I-E molecules of all haplotypes tested, including H-2b, d, f and k, so can be used in all mouse strains.

Réactivité croisée: Souris

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising DA rats with PVG.R8 rat spleen cells. Splenocytes from immunised rats were then isolated, and fused to the myeloma line NSO/u to generate stable hybridomas.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody reacts with murine MHC class II I-A and I-E molecules, as confirmed by two-dimensional gel electrophoresis of Ia immunoprecipitates from metabolically labelled murine lymphoma cells (Momburg et al, 1986). Cross-blocking studies with anti-Ia β-chain MAbs indicate that P7/7 binds to the β-chain of murine Ia (Momburg et al, 1986). This antibody has been used to stain acetone-fixed mouse pancreas sections to study lymphocyte infiltration (Signore et al, 1987, Signore et al, 1989) and ethanol-fixed mouse brain sections (McMenamin et al, 1998). In flow cytometric analysis, this antibody has been employed to identify MHC class II-expressing murine cells (Hayball & Lake, 2004, Heath et al, 1991), and to analyse the surface phenotype of murine N9 microglial cells (Dimayuga et al, 2005). This antibody has been used in immunofluorescence analysis of acetone-fixed thymus sections to identify MHC-II positive and negative medullae (Aschenbrenner et al, 2007).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MHC Class II

Antigène: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Autre désignation: MHC II

Sujet: P7.7, I-A, Ia, I-E, MHC-II, MHC Class II