Recombinant MHC, Class I anticorps

N° du produit ABIN7072424

L’anticorps Lapin Chimeric anti-MHC, Class I a été validé pour IP, IF et IA. Il convient pour détecter MHC, Class I dans des échantillons de Souris.

Aperçu rapide pour Recombinant MHC, Class I anticorps (ABIN7072424)

Antigène: MHC, Class I

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MHC, Class I est non-conjugé

Application: Immunoprecipitation (IP), Immunofluorescence (IF), Immunoassay (IA)

Clone: R1-9-6

Détail du produit anti-MHC, Class I anticorps

Fonction: Anti-MHC I [R1-9.6], Rabbit IgG, kappa

Specificité: This antibody is specific for murine MHC class I, and binds to polymorphic H-2 determinants expressed on most haplotypes. This antibody is, therefore, able to bind all H-2 class I molecules, independent of the allele. This antibody recognises a determinant distinct from those detected by most common mouse monoclonal anti-H-2 antibodies.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Lewis 1F rats with mouse CH-1 lymphoma cells. Spleen cells from immunised rats were fused with X-63Ag8-653 myeloma cells to generate hybridomas.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody binds murine H-2 MHC class I molecules and has been used in both structural and biochemical studies. This antibody has been used in immunoprecipitation analysis (Robinson, 1987) and in immunofluorescence assays for MHC-I surface expression (Karacsonyi et al., 2004) and MHC-I endocytosis (Knorr et al., 2009). This antibody has also been employed to induce clustering of cell surface MHC-I in co-patching assays (Knorr et al., 2009). Cellular radioimmune assays have been used to confirm the binding of this antibody to spleen cells and assess its strain specificity (Koch et al., 1983, PMID: 6192569).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MHC, Class I

Antigène: MHC, Class I

Autre désignation: MHC I

Sujet: R1.9.6, H-2, MHC-I, MHC Class I