Recombinant TIMD2 anticorps (Extracellular Domain)

N° du produit ABIN7072511

L’anticorps anti-TIMD2 Chimeric Lapin est utilisé pour la détection de TIMD2 dans des échantillons de Souris. Il a été validé pour FACS, WB, BP, IHC et Func.

Aperçu rapide pour Recombinant TIMD2 anticorps (Extracellular Domain) (ABIN7072511)

Antigène: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp TIMD2 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Blocking Peptide (BP), Immunohistochemistry (IHC), Functional Studies (Func)

Clone: RMT2-14

Détail du produit anti-TIMD2 anticorps

Épitope: AA 1-230, Extracellular Domain

Fonction: Anti-TIM-2 [RMT2-14], Rabbit IgG, lambda

Specificité: This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.

Isotype: IgG lambda

Information d'application

Indications d'application: This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur TIMD2

Antigène: TIMD2 (T Cell Immunoglobulin and Mucin Domain Containing 2 (TIMD2))

Autre désignation: TIM-2

Sujet: TIMD2, T Cell Immunoglobulin Mucin-2, T Cell immunoglobulin and mucin domain protein 2, TIM3

UniProt: Q8R183