Recombinant Fc epsilon RI/FCER1A anticorps

N° du produit ABIN7072532

L’anticorps Lapin Chimeric anti-Fc epsilon RI/FCER1A a été validé pour FACS, IHC, IP et Dep. Il convient pour détecter Fc epsilon RI/FCER1A dans des échantillons de Souris.

Aperçu rapide pour Recombinant Fc epsilon RI/FCER1A anticorps (ABIN7072532)

Antigène: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Fc epsilon RI/FCER1A est non-conjugé

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Depletion (Dep)

Clone: Mar-1

Détail du produit anti-Fc epsilon RI/FCER1A anticorps

Fonction: Anti-FceR1 alpha [Mar-1], Rabbit IgG, lambda

Specificité: This antibody specifically targets the non-signalling alpha chain of the FceR1 complex.

Attributs du produit:

Original Species of Ab: Hamster, Armenian

Original Format of Ab: IgG

Purification: Protein A affinity purified

Isotype: IgG lambda

Information d'application

Indications d'application: This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004, Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008). Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Armenian Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Fc epsilon RI/FCER1A

Antigène: Fc epsilon RI/FCER1A (FCER1A) (Fc Fragment of IgE Receptor Ia (FCER1A))

Autre désignation: FceR1 alpha

Sujet: Fcer1a, Fc epsilon RI alpha, FceRIa, FceRI alpha, FceRI-α, high affinity IgE receptor, High affinity immunoglobulin epsilon receptor subunit alpha,Fce1a, Fc-epsilon RI-alpha, FcERI, IgE Fc receptor subunit alpha

UniProt: P20489

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process