Recombinant MS4A4A anticorps

N° du produit ABIN7072576

Cet anticorps Lapin Chimeric détecte spécifiquement MS4A4A dans FACS et IP. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant MS4A4A anticorps (ABIN7072576)

Antigène: MS4A4A (Membrane-Spanning 4-Domains, Subfamily A, Member 4 (MS4A4A))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MS4A4A est non-conjugé

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 5C12

Détail du produit anti-MS4A4A anticorps

Fonction: Anti-MS4A4A [5C12], Rabbit IgG, lambda

Specificité: This antibody is specific for human extracellular MS4A4A. 5C12 mAb only partially cross-blocked either 4H2 (Ab01373) and 3F2 (Ab01375) and therefore probably detects a unique or overlapping epitope (Sanyal et al., 2017, pmid: 28303902). MS4A (membrane-spanning 4-domain family, subfamily A) is a large family of proteins that includes at least 26 members in mouse and humans. Flanked by amino- and carboxyl- cytoplasmic regions, MS4A family members contain four highly conserved transmembrane domains connected by short extracellular and cytoplasmic loops. MS4A4A is likely to be a component of oligomeric cell surface complexes involved in signal transduction in diverse cell lineages.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: This antibody was generated by immunizing C57BL/6 mice against mouse cell line 300-19 expressing transfected human membrane-spanning 4-domains subfamily A member 4A (MS4A4A)-GFP.

Isotype: IgG lambda

Information d'application

Indications d'application: 5C12 is recommended for the detection and analysis of expression of MS4A4A. Importantly, MS4A4A has been shown to be a marker for M2 macrophages and plasma cells and to be overexpressed in multiple myelomas, suggesting a potential role in targeting alternatively activated macrophages such as tumor-associated macrophages, and in the treatment of multiple myeloma and mantle cell lymphoma (Sanyal et al., 2017, pmid: 28303902). The antibody 5C12 can be successfully used for the detection of MS4A4A-expressing cells via flow cytometry (Sanyal et al., 2017, pmid: 28303902). Finally, specificity of this antibody was additionally confirmed via immunoprecipitation of lysates of BJAB MS4A4A-HA cells (Sanyal et al., 2017, pmid: 28303902).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MS4A4A

Antigène: MS4A4A (Membrane-Spanning 4-Domains, Subfamily A, Member 4 (MS4A4A))

Autre désignation: MS4A4A

Sujet: Four-span transmembrane protein 1, human membrane-spanning 4-domains subfamily A member 4A, CD20 antigen-like 1, (MS4A4A)-GFP, MS4A4, 4SPAN1, MS4A

UniProt: Q96JQ5